Sonna Larry A, Sawka Michael N, Lilly Craig M
Division of Pulmonary and Critical Care Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Prog Brain Res. 2007;162:321-46. doi: 10.1016/S0079-6123(06)62016-5.
Microarray analysis of gene expression at the level of RNA has generated new insights into the relationship between cellular responses to acute heat shock in vitro, exercise, and exertional heat illness. Here we discuss the systemic physiology of exertional hyperthermia and exertional heat illness, and compare the results of several recent microarray studies performed in vitro on human cells subjected to heat shock and in vivo on samples obtained from subjects performing exercise or suffering from exertional heat injury. From these comparisons, a concept of overlapping component responses emerges. Namely, some of the gene expression changes observed in peripheral blood mononuclear cells during exertional heat injury can be accounted for by normal cellular responses to heat, exercise, or both; others appear to be specific to the disease state itself. If confirmed in future studies, these component responses might provide a better understanding of adaptive and pathological responses to exercise and exercise-induced hyperthermia, help find new ways of identifying individuals at risk for exertional heat illness, and perhaps even help find rational molecular targets for therapeutic intervention.
对RNA水平的基因表达进行微阵列分析，为体外细胞对急性热休克、运动及劳力性热射病的反应之间的关系带来了新的见解。在此，我们讨论劳力性体温过高和劳力性热射病的系统生理学，并比较最近几项微阵列研究的结果，这些研究分别在体外对遭受热休克的人类细胞以及在体内对从进行运动或患有劳力性热损伤的受试者获取的样本进行。通过这些比较，出现了重叠成分反应的概念。也就是说，在劳力性热损伤期间在外周血单核细胞中观察到的一些基因表达变化，可以由细胞对热、运动或两者的正常反应来解释；其他变化似乎是疾病状态本身所特有的。如果在未来研究中得到证实，这些成分反应可能会更好地理解对运动和运动诱导的体温过高的适应性和病理反应，有助于找到识别劳力性热射病高危个体的新方法，甚至可能有助于找到治疗干预的合理分子靶点。
