Ibrahim Hany, Boyer Alexandre, Bouajila Jalloul, Couderc François, Nepveu Françoise
Université de Toulouse - Université Paul Sabatier, UMR 152, IRD, Toulouse 3, 31062, Toulouse Cedex 9, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 15;857(1):59-66. doi: 10.1016/j.jchromb.2007.07.008. Epub 2007 Jul 10.
Non-steroidal anti-inflammatory drugs (NSAIDs) such as piroxicam and mefenamic acid are commonly prescribed to treat inflammation, pain and fever. Similarly acetylsalicylic acid is used to prevent strokes and heart attacks. A rapid and selective method was developed for the simultaneous assay of three NSAIDs and salicylic acid via HPLC with fluorescence detection. The separation was performed using a "dual-mode" gradient (acetonitrile-0.1% aqueous orthophosphoric acid) and the analysis was completed within 7 min using an ACE column C18, 5 microm, 150 mm x 4.6 mm. Naproxen was used as internal standard. The proposed method is simple, selective as well as with a good sensitivity reaching LOD lower than 2 pmol (0.05 microM) and was applied for quantitative analysis in pharmaceuticals and in human serum samples. The mean recovery was more than 95% and the within-day and between-days precisions were found to be satisfactory having RSD within the acceptable limits (<10%).
非甾体抗炎药（NSAIDs）如吡罗昔康和甲芬那酸通常用于治疗炎症、疼痛和发热。同样，乙酰水杨酸用于预防中风和心脏病发作。开发了一种快速且选择性的方法，通过高效液相色谱-荧光检测同时测定三种非甾体抗炎药和水杨酸。使用“双模式”梯度（乙腈-0.1%正磷酸水溶液）进行分离，并使用ACE C18柱（5微米，150毫米×4.6毫米）在7分钟内完成分析。萘普生用作内标。所提出的方法简单、选择性好，灵敏度高，检测限低于2皮摩尔（0.05微摩尔），并应用于药物和人血清样品的定量分析。平均回收率超过95%，日内和日间精密度令人满意，相对标准偏差在可接受范围内（<10%）。
