He Chong-Bo, Cao Jie, Liu Wei-Dong, Zhou Zun-Chun, Ge Long-Li, Gao Xiang-Gang, Wang Xiao-Min
Liaoning Ocean and Fisheries Science Research Institute, Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Open Lab of Applied Marine Biotechnology, Dalian 116023 China.
Yi Chuan. 2007 Jul;29(7):829-36. doi: 10.1360/yc-007-0829.
Spotted halibut (Verasper variegatus) is the only species of Genus Verasper in China. The fish was naturally distributed in Yellow Sea and Bohai Sea in northern China and Kyushu in Japan and in Korean sea area. Using PCR product direct sequencing, mitochondrial control region sequences of 24 individuals of spotted halibut was confirmed and analyzed. 4 control region haplotypes, resulting from length heteroplamy of the tandem repeat region, was obtained from these 24 fish. Sequence analysis demonstrated that there were four similar structures in the control region, i.e., extended terminal associated sequences (ETAS), central conserved sequence block (CSB), conserved sequence block (CSB), and repeat region, in V. moseri, Limanda ferruginea, Reinhardtius hippoglossoides, Heppoglossoides platessoides, Paralichthys olivaceous, Solea solea, S. senegalensis, and S. lascari. By comparing with other vertebrates, we found that there were similar repeated sequences immediately after the CSB-3 in all of the anuran species.
星斑川鲽（Verasper variegatus）是中国鲽属的唯一物种。该鱼自然分布于中国北方的黄海和渤海以及日本九州和韩国海域。采用PCR产物直接测序法，对24尾星斑川鲽个体的线粒体控制区序列进行了测定和分析。从这24尾鱼中获得了由串联重复区长度异质性产生的4种控制区单倍型。序列分析表明，在莫氏星鲽、锈赤星鲽、大西洋庸鲽、太平洋庸鲽、绿鳍马面鲀、欧洲鳎、塞内加尔鳎和拉斯卡鳎的控制区中存在四种相似结构，即延伸末端相关序列（ETAS）、中央保守序列块（CSB）、保守序列块（CSB）和重复区。通过与其他脊椎动物比较，我们发现在所有无尾目物种中，CSB - 3之后紧邻着相似的重复序列。
