He Chongbo, Han Jiabo, Ge Longli, Zhou Zunchun, Gao Xianggang, Mu Yunlei, Liu Weidong, Cao Jie, Liu Zhanjiang
Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, People's Republic of China.
DNA Seq. 2008 Jun;19(3):246-55. doi: 10.1080/10425170701563303.
In this work, the mitochondrial genomes for spotted halibut (Verasper variegatus) and barfin flounder (Verasper moseri) were completely sequenced. The entire mitochondrial genome sequences of the spotted halibut and barfin flounder were 17,273 and 17,588 bp in length, respectively. The organization of the two mitochondrial genomes was similar to those reported from other fish mitochondrial genomes containing 37 genes (2 rRNAs, 22 tRNAs and 13 protein-coding genes) and two non-coding regions (control region (CR) and WANCY region). In the CR, the termination associated sequence (ETAS), six central conserved block (CSB-A,B,C,D,E,F), three conserved sequence blocks (CSB1-3) and a region of 61-bp tandem repeat cluster at the end of CSB-3 were identified by similarity comparison with fishes and other vertebrates. The tandem repeat sequences show polymorphism among the different individuals of the two species. The complete mitochondrial genomes of spotted halibut and barfin flounder should be useful for evolutionary studies of flatfishes and other vertebrate species.
在本研究中,对斑头鲽(Verasper variegatus)和条斑星鲽(Verasper moseri)的线粒体基因组进行了全序列测定。斑头鲽和条斑星鲽的线粒体基因组全序列长度分别为17,273和17,588碱基对。这两个线粒体基因组的结构与其他鱼类线粒体基因组相似,包含37个基因(2个rRNA、22个tRNA和13个蛋白质编码基因)以及两个非编码区(控制区(CR)和WANCY区)。在控制区中,通过与鱼类和其他脊椎动物的相似性比较,鉴定出了终止相关序列(ETAS)、六个中央保守块(CSB-A、B、C、D、E、F)、三个保守序列块(CSB1-3)以及CSB-3末端的一个61碱基对串联重复簇。串联重复序列在这两个物种的不同个体间表现出多态性。斑头鲽和条斑星鲽的线粒体基因组全序列应有助于比目鱼和其他脊椎动物物种的进化研究。