Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China.
Mol Biol Rep. 2011 Oct;38(7):4545-51. doi: 10.1007/s11033-010-0585-1. Epub 2010 Dec 4.
Barfin flounder (Verasper moseri) and spotted halibut (Verasper variegatus) are two commercially important flatfish species in the Northeast Asia. In the present study, we reported polymorphic microsatellite markers in V. moseri and V. variegatus by the cross-species amplification of microsatellite primers developed previously in two other related marine fish species. A total of 244 polymorphic microsatellite markers were selected for cross-species amplification in V. moseri and V. variegatus, of which 182 markers deriving from Atlantic halibut (Hippoglossus hippoglossus) and 62 markers deriving from Japanese flounder (Paralichthys olivaceus). A sample of 10 individuals were detected. As a result, a total of 67 loci showed polymorphisms in V. moseri, and 62 loci showed polymorphisms in V. variegatus, with the observed number of alleles per locus ranging from two to five in V. moseri, and from two to seven in V. variegatus, respectively. This paper provided more candidate microsatellite markers which could be useful for construction of genetic linkage maps, evaluation of population genetic structure and stock management of V. moseri and V. variegatus.
圆斑星鲽（Verasper moseri）和星斑川鲽（Verasper variegatus）是东北亚地区两种重要的商业性比目鱼类。本研究通过交叉物种扩增先前在两种其他相关海洋鱼类中开发的微卫星引物，报道了 V. moseri 和 V. variegatus 中的多态性微卫星标记。共选择了 244 个多态性微卫星标记用于 V. moseri 和 V. variegatus 的交叉物种扩增，其中 182 个标记来自大西洋比目鱼（Hippoglossus hippoglossus），62 个标记来自日本牙鲆（Paralichthys olivaceus）。对 10 个个体进行了检测。结果，在 V. moseri 中共检测到 67 个位点具有多态性，在 V. variegatus 中共检测到 62 个位点具有多态性，每个位点的观察等位基因数在 V. moseri 中从 2 到 5 不等，在 V. variegatus 中从 2 到 7 不等。本文提供了更多的候选微卫星标记，可用于构建遗传连锁图谱、评估种群遗传结构和圆斑星鲽和星斑川鲽的种群管理。
