Cary J W, OBrian G R, Nielsen D M, Nierman W, Harris-Coward P, Yu J, Bhatnagar D, Cleveland T E, Payne G A, Calvo A M
Southern Regional Research Center,Agricultural Research Service, U.S. Department of Agriculture, New Orleans, LA, USA.
Appl Microbiol Biotechnol. 2007 Oct;76(5):1107-18. doi: 10.1007/s00253-007-1081-y. Epub 2007 Jul 24.
The aflatoxin-producing fungi, Aspergillus flavus and A. parasiticus, form structures called sclerotia that allow for survival under adverse conditions. Deletion of the veA gene in A. flavus and A. parasiticus blocks production of aflatoxin as well as sclerotial formation. We used microarray technology to identify genes differentially expressed in wild-type veA and veA mutant strains that could be involved in aflatoxin production and sclerotial development in A. flavus. The DNA microarray analysis revealed 684 genes whose expression changed significantly over time; 136 of these were differentially expressed between the two strains including 27 genes that demonstrated a significant difference in expression both between strains and over time. A group of 115 genes showed greater expression in the wild-type than in the veA mutant strain. We identified a subgroup of veA-dependent genes that exhibited time-dependent expression profiles similar to those of known aflatoxin biosynthetic genes or that were candidates for involvement in sclerotial production in the wild type.
产黄曲霉毒素的真菌，黄曲霉和寄生曲霉，会形成称为菌核的结构，使其能够在不利条件下存活。黄曲霉和寄生曲霉中veA基因的缺失会阻止黄曲霉毒素的产生以及菌核的形成。我们使用微阵列技术来鉴定在野生型veA和veA突变菌株中差异表达的基因，这些基因可能参与黄曲霉的黄曲霉毒素产生和菌核发育。DNA微阵列分析揭示了684个基因，其表达随时间发生显著变化；其中136个在两个菌株之间差异表达，包括27个在菌株之间以及随时间表达均有显著差异的基因。一组115个基因在野生型中的表达高于veA突变菌株。我们鉴定出一个veA依赖性基因亚组，其表现出与已知黄曲霉毒素生物合成基因相似的时间依赖性表达谱，或者是野生型中参与菌核产生的候选基因。
