Mayser W, Betz H, Schloss P
Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.
FEBS Lett. 1991 Dec 16;295(1-3):203-6. doi: 10.1016/0014-5793(91)81418-8.
Degenerate oligonucleotides deduced from two regions of the rat gamma-aminobutyric acid (GABA) transporter were used to amplify related sequences from rat spinal cord by polymerase chain reaction (PCR). The resulting product then allowed isolation of two overlapping cDNA clones with an open reading frame encoding a hydrophobic polypeptide of 630 amino acids. This protein, termed NTT, exhibits 38% sequence homology to the GABA and 48% to the noradrenaline transporters and contains 12 putative transmembrane regions. PCR amplification revealed low-level expression of NTT transcripts in spinal cord, but not in brain and cerebellum.
从大鼠γ-氨基丁酸（GABA）转运体的两个区域推导出来的简并寡核苷酸，被用于通过聚合酶链反应（PCR）从大鼠脊髓中扩增相关序列。随后得到的产物使得能够分离出两个重叠的cDNA克隆，其开放阅读框编码一个由630个氨基酸组成的疏水多肽。这种蛋白质，称为NTT，与GABA转运体有38%的序列同源性，与去甲肾上腺素转运体有48%的序列同源性，并且含有12个推定的跨膜区域。PCR扩增显示NTT转录本在脊髓中有低水平表达，但在脑和小脑中没有表达。
