Laboratório de Virologia, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, AM, Brasil.
Rev Soc Bras Med Trop. 2012 Jun;45(3):393-4. doi: 10.1590/s0037-86822012000300022.
Manaus, the capital city of the state of Amazon with nearly 2 million inhabitants, is located in the middle of the Amazon rain forest and has suffered dengue outbreaks since 1998.
In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying dengue virus serotypes.
Acute phase sera from 432 patients were tested for the presence of dengue virus. Out of the 432 patients, 137 (31.3%) were found to be positive. All the four dengue virus serotypes were observed.
The simultaneous circulation of the four dengue serotypes is described for the first time in Manaus and in Brazil.
玛瑙斯是亚马逊州首府，拥有近 200 万居民，位于亚马逊雨林中心，自 1998 年以来爆发了登革热疫情。
本研究采用逆转录聚合酶链反应（RT-PCR）对血液样本进行调查，旨在鉴定登革热病毒血清型。
对 432 名患者的急性期血清进行了登革热病毒检测，其中 137 例（31.3%）呈阳性。观察到了所有四种登革热病毒血清型。
这是首次在玛瑙斯和巴西描述四种登革热血清型的同时传播。
