Korgun E T, Cayli S, Asar M, Demir R
Department of Histology and Embryology, Akdeniz University Medical Faculty, Campus, Antalya 07070, Turkey.
J Mol Histol. 2007 Aug;38(4):253-60. doi: 10.1007/s10735-007-9095-4. Epub 2007 May 22.
The aim of this study was to investigate the immunohistochemical distribution of laminin, vimentin and desmin during the implantation period in the rat since ECM remodelling and the expression of intermediate filaments (Ifs) is essential for successful decidualization and implantation. On day 4 of pregnancy, laminin was found in a few endometrial stromal cells (ESC), the basement membrane of the numerous endometrial blood vessels, in endometrial glands and as well as in the uterine epithelium. The localization of vimentin on day 4 of pregnancy was widespread in the ESC. However, desmin immunoreactivity was low in ESC on this day of pregnancy. On day 6 of pregnancy, laminin and vimentin were localized in the decidual area underlying luminal epithelium and around the implanting embryo. Additionally, desmin was found to be present densely in decidual cells of the anti-mesometrial region where implantation takes place. Finally, on day 8 of pregnancy, laminin was present in decidual and parietal endodermal cells, whereas vimentin was immunolocalized in primary and secondary decidual regions in the endometrium. In contrast, desmin was detected in some parts of the secondary decidual zone. In conclusion, these proteins could have crucial roles in decidualization and implantation.
本研究的目的是调查层粘连蛋白、波形蛋白和结蛋白在大鼠植入期的免疫组织化学分布，因为细胞外基质重塑和中间丝（IFs）的表达对于成功的蜕膜化和植入至关重要。在妊娠第4天，层粘连蛋白存在于少数子宫内膜基质细胞（ESC）、众多子宫内膜血管的基底膜、子宫内膜腺体以及子宫上皮中。妊娠第4天波形蛋白的定位在ESC中广泛分布。然而，在妊娠的这一天，ESC中的结蛋白免疫反应性较低。在妊娠第6天，层粘连蛋白和波形蛋白位于腔上皮下方的蜕膜区域以及植入胚胎周围。此外，发现结蛋白密集存在于发生植入的子宫系膜对侧区域的蜕膜细胞中。最后，在妊娠第8天，层粘连蛋白存在于蜕膜细胞和壁内胚层细胞中，而波形蛋白免疫定位在子宫内膜的初级和次级蜕膜区域。相比之下，在次级蜕膜区的一些部位检测到了结蛋白。总之，这些蛋白质可能在蜕膜化和植入中起关键作用。
