Drowart A, Launois P, De Cock M, Huygen K, De Bruyn J, Yernault J C, Van Vooren J P
Chest Department, Erasme Hospital, Brussels, Belgium.
J Immunol Methods. 1991 Dec 15;145(1-2):223-8. doi: 10.1016/0022-1759(91)90330-i.
Isoelectric focusing was used to separate the three components of the antigen 85 complex of Mycobacterium bovis BCG. Antibody responses of leprosy patients against each Mycobacterium bovis BCG. Antibody responses of leprosy patients against each component were quantitated by densitometric analysis of immunoblot assays. The 85A component was recognized by 40% (8/20) of the lepromin positive and negative healthy subjects, by 76% (19/25) of the tuberculoid and by 96% (24/25) of the lepromatous leprosy sera. In contrast, the 85B component was not stained by the control sera, nor by the tuberculoid leprosy sera but by 64% (16/25) of the lepromatous leprosy sera. The results suggest that antigen 85B contains one or several epitopes that are specifically recognized by sera of lepromatous leprosy patients only.
采用等电聚焦法分离卡介苗（Mycobacterium bovis BCG）抗原85复合物的三种成分。对麻风病人针对每种卡介苗成分的抗体反应进行了研究。通过免疫印迹分析的光密度测定法定量麻风病人针对每种成分的抗体反应。85A成分在40%（8/20）的麻风菌素阳性和阴性健康受试者血清、76%（19/25）的结核样型麻风病人血清以及96%（24/25）的瘤型麻风病人血清中被识别。相比之下，85B成分在对照血清和结核样型麻风病人血清中未被染色，但在64%（16/25）的瘤型麻风病人血清中被染色。结果表明，抗原85B含有一个或几个仅被瘤型麻风病人血清特异性识别的表位。
