Jiang Wen, Chiu Wah
Department of Biological Sciences, Purdue Univesity, IN, USA.
Methods Mol Biol. 2007;369:345-63. doi: 10.1007/978-1-59745-294-6_17.
With the rapid progresses in both instrumentation and computing, it is increasingly straightforward and routine to determine the structures of icosahedral viruses to subnanometer resolutions (6-10 A) by cryoelectron microscopy and image reconstruction. In this resolution range, secondary structure elements of protein subunits can be clearly discerned. Combining the three-dimensional density map and bioinformatics of the protein components, the folds of the virus capsid shell proteins can be derived. This chapter will describe the experimental and computational procedures that lead to subnanometer resolution structural determinations of icosahedral virus particles. In addition, we will describe how to extract useful structural information from the three-dimensional maps.
随着仪器设备和计算技术的飞速发展，通过冷冻电子显微镜和图像重建技术将二十面体病毒的结构测定到亚纳米分辨率（6 - 10埃）变得越来越直接和常规。在这个分辨率范围内，可以清晰地辨别蛋白质亚基的二级结构元件。结合蛋白质组分的三维密度图和生物信息学，可以推导病毒衣壳壳蛋白的折叠结构。本章将描述实现二十面体病毒颗粒亚纳米分辨率结构测定的实验和计算过程。此外，我们还将描述如何从三维图谱中提取有用的结构信息。
