高分辨率 3D 结构揭示了呼肠孤病毒的生物学功能。
High-resolution 3D structures reveal the biological functions of reoviruses.
机构信息
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
出版信息
Virol Sin. 2013 Dec;28(6):318-25. doi: 10.1007/s12250-013-3341-6. Epub 2013 Nov 6.
Abstract
Viruses in the family Reoviridae are non-enveloped particles comprising a segmented double-stranded RNA genome surrounded by a two-layered or multi-layered icosahedral protein capsid. These viruses are classified into two sub-families based on their particle structural organization. Recent studies have focused on high-resolution three-dimensional structures of reovirus particles by using cryo-electron microscopy (cryo-EM) to approach the resolutions seen in X-ray crystallographic structures. The results of cryo-EM image reconstructions allow tracing of most of the protein side chains, and thus permit integration of structural and functional information into a coherent mechanism for reovirus assembly and entry.
摘要
呼肠孤病毒科的病毒是非包膜粒子，由分段的双链 RNA 基因组和双层或多层二十面体蛋白衣壳组成。这些病毒根据其粒子结构组织被分为两个亚科。最近的研究通过使用冷冻电镜 (cryo-EM) 来达到 X 射线晶体结构的分辨率，重点关注呼肠孤病毒粒子的高分辨率三维结构。冷冻电镜图像重建的结果允许追踪大多数蛋白质侧链，从而将结构和功能信息整合到呼肠孤病毒组装和进入的连贯机制中。
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