State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Virol Sin. 2013 Dec;28(6):318-25. doi: 10.1007/s12250-013-3341-6. Epub 2013 Nov 6.
Viruses in the family Reoviridae are non-enveloped particles comprising a segmented double-stranded RNA genome surrounded by a two-layered or multi-layered icosahedral protein capsid. These viruses are classified into two sub-families based on their particle structural organization. Recent studies have focused on high-resolution three-dimensional structures of reovirus particles by using cryo-electron microscopy (cryo-EM) to approach the resolutions seen in X-ray crystallographic structures. The results of cryo-EM image reconstructions allow tracing of most of the protein side chains, and thus permit integration of structural and functional information into a coherent mechanism for reovirus assembly and entry.
呼肠孤病毒科的病毒是非包膜粒子，由分段的双链 RNA 基因组和双层或多层二十面体蛋白衣壳组成。这些病毒根据其粒子结构组织被分为两个亚科。最近的研究通过使用冷冻电镜 (cryo-EM) 来达到 X 射线晶体结构的分辨率，重点关注呼肠孤病毒粒子的高分辨率三维结构。冷冻电镜图像重建的结果允许追踪大多数蛋白质侧链，从而将结构和功能信息整合到呼肠孤病毒组装和进入的连贯机制中。
