Wang Xiaoyan, Su Mingming, Qiu Yunping, Ni Yan, Zhao Tie, Zhou Mingmei, Zhao Aihua, Yang Shengli, Zhao Liping, Jia Wei
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
J Proteome Res. 2007 Sep;6(9):3449-55. doi: 10.1021/pr070051w. Epub 2007 Jul 21.
Acute stress may trigger systemic biochemical and physiological changes in living organisms, leading to a rapid loss of homeostasis, which can be gradually reinstated by self-regulatory mechanisms and/or drug intervention strategy. However, such a sophisticated metabolic regulatory process has so far been poorly understood, especially from a holistic view. Urinary metabolite profiling of Sprague-Dawley rats exposed to cold temperature (-10 degrees C) for 2 h using GC/MS in conjunction with modern multivariate statistical techniques revealed drastic biochemical changes as evidenced by fluctuations of urinary metabolites and demonstrated the protective effect of total ginsenosides (TGs) in ginseng extracts on stressed rats. The metabonomics approach enables us to visualize significant alterations in metabolite expression patterns as a result of stress-induced metabolic responses and post-stress compensation, and drug intervention. Several major metabolic pathways including catecholamines, glucocorticoids, the tricarboxylic acid (TCA) cycle, tryptophan (nicotinate), and gut microbiota metabolites were identified to be involved in metabolic regulation and compensation required to restore homeostasis.
急性应激可能会触发生物体全身性的生化和生理变化，导致体内平衡迅速丧失，而这种平衡可通过自我调节机制和/或药物干预策略逐渐恢复。然而，迄今为止，人们对这样一个复杂的代谢调节过程了解甚少，尤其是从整体角度来看。利用气相色谱/质谱联用技术（GC/MS）结合现代多元统计技术，对暴露于低温（-10摄氏度）2小时的斯普拉格-道利大鼠进行尿液代谢物谱分析，结果显示尿液代谢物波动表明发生了剧烈的生化变化，并证明人参提取物中的总人参皂苷（TGs）对应激大鼠具有保护作用。代谢组学方法使我们能够观察到由于应激诱导的代谢反应、应激后补偿以及药物干预导致的代谢物表达模式的显著变化。已确定包括儿茶酚胺、糖皮质激素、三羧酸（TCA）循环代谢、色氨酸（烟酸）和肠道微生物群代谢物在内的几个主要代谢途径参与了恢复体内平衡所需的代谢调节和补偿过程。
