通过16S rDNA片段的变性梯度凝胶电泳比较两个加利福尼亚河口浮游细菌的系统发育组成。
Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments.
作者信息
Murray A E, Hollibaugh J T, Orrego C
机构信息
Center for Environmental Studies, San Francisco State University, Tiburon, California 94920, USA.
出版信息
Appl Environ Microbiol. 1996 Jul;62(7):2676-80. doi: 10.1128/aem.62.7.2676-2680.1996.
Abstract
The phylogenetic compositions of bacterioplankton assemblages from San Francisco Bay and Tomales Bay, Calif., differed substantially when analyzed by PCR-denaturing gradient gel electrophoresis; these differences are consistent with the results of previous studies demonstrating differences in their metabolic capabilities. PCR-denaturing gradient gel electrophoresis analysis of complex microbial assemblages was sensitive and reliable, and the results were reproducible as shown by experiments with constructed and naturally occurring assemblages.
摘要
利用聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）分析加利福尼亚州旧金山湾和托马莱斯湾浮游细菌群落的系统发育组成时，二者存在显著差异；这些差异与先前研究中表明它们代谢能力存在差异的结果一致。对复杂微生物群落进行PCR-DGGE分析既灵敏又可靠，构建群落和自然群落实验结果表明，该分析结果具有可重复性。
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