Zhang Rui, Lau Stanley C K, Ki Jang-Seu, Thiyagarajan Vengatesen, Qian Pei-Yuan
Coastal Marine Laboratory, Department of Biology, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
FEMS Microbiol Ecol. 2009 Sep;69(3):449-60. doi: 10.1111/j.1574-6941.2009.00726.x. Epub 2009 Jun 22.
Bacterioplankton community structures under contrasting subtropical marine environments (Hong Kong waters) were analyzed using 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing of predominant bands for samples collected bimonthly from 2004 to 2006 at five stations. Generally, bacterial abundance was significantly higher in the summer than in the winter. The general seasonal variations of the bacterial community structure, as indicated by cluster analysis of the DGGE pattern, were best correlated with temperature at most stations, except for the station close to a sewage discharge outfall, which was best explained by pollution-indicating parameters (e.g. biochemical oxygen demand). Anthropogenic pollutions appear to have affected the presence and the intensity of DGGE bands at the stations receiving discharge of primarily treated sewage. The relative abundance of major bacterial species, calculated by the relative intensity of DGGE bands after PCR amplification, also indicated the effects of hydrological or seasonal variations and sewage discharges. For the first time, a systematic molecular fingerprinting analysis of the bacterioplankton community composition was carried out along the environmental and pollution gradient in a subtropical marine environment, and it suggests that hydrological conditions and anthropogenic pollutions altered the total bacterial community as well as the dominant bacterial groups.
利用16S rRNA基因变性梯度凝胶电泳（DGGE）以及对2004年至2006年每两个月从五个站点采集的样本的主要条带进行后续测序，分析了亚热带海洋环境（香港水域）形成对比的情况下浮游细菌群落结构。总体而言，夏季的细菌丰度显著高于冬季。DGGE图谱聚类分析表明，大多数站点细菌群落结构的总体季节变化与温度的相关性最好，但靠近污水排放口的站点除外，该站点的变化用污染指示参数（如生化需氧量）解释最为合适。人为污染似乎影响了接收初级处理污水排放的站点DGGE条带的存在和强度。通过PCR扩增后DGGE条带的相对强度计算得出的主要细菌种类的相对丰度，也表明了水文或季节变化以及污水排放的影响。首次在亚热带海洋环境中沿着环境和污染梯度对浮游细菌群落组成进行了系统的分子指纹分析，结果表明水文条件和人为污染改变了总细菌群落以及优势细菌类群。
