Aziz Faissal, Parrado Rubio Juan, Ouazzani Naaila, Dary Mohammed, Manyani Hamid, Rodríguez Morgado Bruno, Mandi Laila
National Center for Research and Studies on Water and Energy, University Cadi Ayyad, Marrakech, Morocco.
Laboratory of Hydrobiology, Ecotoxicology & Sanitation (LHEA, URAC 33), Faculty of Sciences Semlalia, Marrakech, Morocco.
Saudi J Biol Sci. 2017 May;24(4):767-777. doi: 10.1016/j.sjbs.2016.01.034. Epub 2016 Jan 25.
In Morocco, storage reservoirs are particular systems of water supply in rural areas. These reservoirs are fed with rainwater and/or directly from the river, which are very contaminated by several pathogenic bacteria. They are used without any treatment as a drinking water by the surrounding population. In this context, the aim of this study is to evaluate the impact of consuming contaminated water stored in reservoirs on health status for six rural communities located in Assif El Mal, Southern East of Marrakech. This was investigated using a classical methodology based on population survey and by molecular approach using PCR-DGGE technique to determine the intestinal bacterial diversity of consumers. The survey showed that, the residents of the studied area suffered from numerous health problems (diarrheal diseases, vomiting or hepatitis A) due to the lack of waste management infrastructures. The consumer's stool analysis by molecular approach revealed that numbers of , and , were significantly higher in the diarrheal feces. In addition, PCR-DGGE study of the prevalence and distribution of bacteria causing human diseases, confirmed that, there is a relationship between water bacterial contaminations of storage reservoirs and microbial disease related health status. Therefore, water reservoir consumption is assumed to be the mean way of exposure for this population. It's clear that this approach gives a very helpful tool to confirm without any doubt the relationship between water bacterial contamination and health status.
在摩洛哥，蓄水水库是农村地区特殊的供水系统。这些水库由雨水和/或直接从河流取水补给，受到多种致病细菌的严重污染。周边居民将其未经任何处理就用作饮用水。在此背景下，本研究的目的是评估饮用储存在水库中的受污染水对马拉喀什东南部阿西夫·埃尔·马尔六个农村社区居民健康状况的影响。本研究采用基于人口调查的经典方法，并通过使用PCR-DGGE技术的分子方法来确定消费者肠道细菌的多样性。调查显示，由于缺乏废物管理基础设施，研究区域的居民患有多种健康问题（腹泻病、呕吐或甲型肝炎）。通过分子方法对消费者粪便进行分析发现，腹泻粪便中的 、 和 的数量显著更高。此外，对引起人类疾病的细菌的流行情况和分布进行的PCR-DGGE研究证实，蓄水水库的水细菌污染与微生物疾病相关的健康状况之间存在关联。因此，饮用蓄水水库的水被认为是该人群的主要接触途径。显然，这种方法提供了一个非常有用的工具，毫无疑问地证实了水细菌污染与健康状况之间的关系。
