通过光开关发射体的异步定位实现全细胞荧光纳米显微镜技术。

Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters.

作者信息

Egner Alexander, Geisler Claudia, von Middendorff Claas, Bock Hannes, Wenzel Dirk, Medda Rebecca, Andresen Martin, Stiel Andre C, Jakobs Stefan, Eggeling Christian, Schönle Andreas, Hell Stefan W

机构信息

Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.

出版信息

Biophys J. 2007 Nov 1;93(9):3285-90. doi: 10.1529/biophysj.107.112201. Epub 2007 Jul 27.

Abstract

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.

摘要

我们通过可逆光开关以及在相对较快的记录速度下对单个荧光团进行定位,并从完整细胞内部实现了远场荧光显微镜的纳米级分辨率。通过异步记录单个分子开关周期的光子爆发,这些进展成为可能。我们展示了来自完整哺乳动物细胞微管网络的图像,分辨率为40纳米。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b24/2025649/b67695fdb7e4/biophysj00112201F01_HT.jpg

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