Nishikawa Satomi, Yasoshima Akira, Doi Kunio, Nakayama Hiroyuki, Uetsuka Koji
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo 113-8657, Japan.
Exp Anim. 2007 Jul;56(4):263-72. doi: 10.1538/expanim.56.263.
Although a number of obesity animal models have been reported, each model possesses different characteristics of obesity, suggesting care should be taken in choosing an animal model suitable for the experimental purpose. In this report, we fed 4-(young) and 52-week-old (middle-aged) C57BL/6J (B6) and young BALB/cA (BALB/c) mice with a high fat diet (HFD) for 9 weeks, and investigated the clinical and histological characteristics of obesity. In BALB/c mice, males gained more body weight and body fat weight and had higher energy intake than females by HFD feeding. Comparing the effect of HFD feeding between the strains of mice, BALB/c male mice accumulated more hepatic lipid than B6 male mice. In addition, middle-aged B6 mice increased the ratio of fat to body weight and hepatic lipid accumulation more than young mice. In conclusion, the characteristics of obesity induced by HFD feeding were influenced by the sex, strain and age of mice. Sex steroid hormones, hepatic lipid metabolism and systemic metabolism might be involved in these factors. The basic data in this study will be useful for the development of animal models of high fat diet-induced obesity.
尽管已经报道了多种肥胖动物模型，但每种模型都具有不同的肥胖特征，这表明在选择适合实验目的的动物模型时应谨慎。在本报告中，我们用高脂饮食（HFD）喂养4周龄（年轻）和52周龄（中年）的C57BL/6J（B6）小鼠以及年轻的BALB/cA（BALB/c）小鼠9周，并研究了肥胖的临床和组织学特征。在BALB/c小鼠中，通过高脂饮食喂养，雄性小鼠比雌性小鼠体重增加更多、体脂重量更高且能量摄入更高。比较不同品系小鼠高脂饮食喂养的效果，BALB/c雄性小鼠比B6雄性小鼠积累了更多的肝脏脂质。此外，中年B6小鼠比年轻小鼠体脂与体重的比值增加更多，肝脏脂质积累也更多。总之，高脂饮食诱导的肥胖特征受小鼠的性别、品系和年龄影响。这些因素可能涉及性类固醇激素、肝脏脂质代谢和全身代谢。本研究中的基础数据将有助于高脂饮食诱导肥胖动物模型的开发。
