Likhoded V G, Kuleshova N V, Sergieva N V, Konev Iu V, Trubnikova I A, Sudzhian E V
Zh Mikrobiol Epidemiol Immunobiol. 2007 May-Jun(3):3-6.
Method of Gram-negative bacteria endotoxins detection on the basis of their own spectrum of electromagnetic radiation frequency was developed. Frequency spectrum typical for chemotype Re glycolipid, which is a part of lypopolysaccharides in the majority of Gram-negative bacteria, was used. Two devices--"Mini- Expert-DT" (manufactured by IMEDIS, Moscow) and "Bicom" (manufactured by Regumed, Germany)--were used as generators of electromagnetic radiation. Detection of endotoxin using these devices was performed by electropuncture vegetative resonance test. Immunoenzyme reaction with antibodies to chemotype Re glycolipid was used during analysis of preparations for assessment of resonance-frequency method specificity. The study showed that resonance-frequency method can detect lypopolysaccharides of different enterobacteria in quantities up to 0.1 pg as well as bacteria which contain lypopolysaccharides. At the same time, this method does not detect such bacteria as Staphylococcus aureus, Bifidobacterium spp., Lactobacillus spp., and Candida albicans. The method does not require preliminary processing of blood samples and can be used for diagnostics of endotoxinemia, and detection of endotoxins in blood samples or injection solutions.
基于革兰氏阴性菌自身电磁辐射频率谱开发了其内毒素检测方法。使用了大多数革兰氏阴性菌脂多糖一部分的Re化学型糖脂典型的频谱。使用了两种设备——“Mini-Expert-DT”（由莫斯科的IMEDIS制造）和“Bicom”（由德国的Regumed制造）作为电磁辐射发生器。使用这些设备通过电针植物性共振试验检测内毒素。在分析制剂以评估共振频率法特异性时，使用了与Re化学型糖脂抗体的免疫酶反应。研究表明，共振频率法能够检测出含量低至0.1 pg的不同肠杆菌脂多糖以及含有脂多糖的细菌。同时，该方法检测不出金黄色葡萄球菌、双歧杆菌属、乳杆菌属和白色念珠菌等细菌。该方法不需要对血样进行预处理，可用于内毒素血症的诊断以及血样或注射溶液中内毒素的检测。
