Travagli Valter, Zanardi Iacopo, Silvietti Antonella, Bocci Velio
Department of Pharmaceutical Chemistry and Technology, University of Siena, Siena, Italy.
Int J Biol Macromol. 2007 Dec 1;41(5):504-11. doi: 10.1016/j.ijbiomac.2007.06.010. Epub 2007 Jun 30.
Ozonation of either human whole blood or saline-washed erythrocytes causes considerable damage to the latter and this result has opened a controversy. With the benefit of hindsight, it appears logical that once erythrocytes are deprived of the potent antioxidants of plasma, they become very sensitive to the oxidant effects of ozone. The aim of the present work was to perform a physical-chemical evaluation of some critical parameters able to clarify this issue. We have ascertained that when whole blood is exposed to the appropriate ozone doses used in human therapy, no damage ensues while saline-washed erythrocytes undergo conspicuous haemolysis. The dogma that ozone is always toxic is incorrect because its reactivity below the concentration of 80mug/mL can be controlled by the plasmatic antioxidant system.
对人全血或生理盐水洗涤过的红细胞进行臭氧处理会对后者造成相当大的损伤，这一结果引发了一场争论。事后看来，一旦红细胞被剥夺了血浆中的强效抗氧化剂，它们就会对臭氧的氧化作用变得非常敏感，这似乎是合乎逻辑的。本研究的目的是对一些关键参数进行物理化学评估，以阐明这一问题。我们已经确定，当全血暴露于人类治疗中使用的适当臭氧剂量时，不会产生损伤，而生理盐水洗涤过的红细胞则会发生明显的溶血。认为臭氧总是有毒的教条是不正确的，因为在浓度低于80微克/毫升时，其反应性可以由血浆抗氧化系统控制。
