Naivar Mark A, Parson Jimmie D, Wilder Mark E, Habbersett Robert C, Edwards Bruce S, Sklar Larry, Nolan John P, Graves Steven W, Martin John C, Jett James H, Freyer James P
Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Cytometry A. 2007 Nov;71(11):915-24. doi: 10.1002/cyto.a.20445.
A digital signal processing (DSP)-based digital data acquisition system has been developed to support novel flow cytometry efforts. The system flexibility includes how it detects, captures, and processes event data. Custom data capture boards utilizing analog to digital converters (ADCs) and field programmable gate arrays (FPGA) detect events and capture correlated event data. A commercial DSP board processes the captured data and sends the results over the IEEE 1394 bus to the host computer that provides a user interface for acquisition, display, analysis, and storage. The system collects list mode data, correlated pulse shapes, or streaming data from a variety of detector types using Linux, Mac OS X, and Windows host computers. It extracts pulse features not found on commercial systems with excellent sensitivity and linearity over a wide dynamic range. List mode data are saved in FCS 3.0 formatted files while streaming or correlated waveform data are saved in custom format files for postprocessing. Open, reconfigurable cytometric acquisition system is compact, scaleable, flexible, and modular. Programmable feature extraction algorithms have exciting possibilities for both new and existing applications. The recent availability of a commercial data capture board will enable general availability of similar systems.
一种基于数字信号处理（DSP）的数字数据采集系统已被开发出来，以支持新型流式细胞术研究。该系统的灵活性体现在其检测、捕获和处理事件数据的方式上。利用模数转换器（ADC）和现场可编程门阵列（FPGA）的定制数据捕获板检测事件并捕获相关事件数据。一块商用DSP板处理捕获到的数据，并通过IEEE 1394总线将结果发送到主机，主机提供用于采集、显示、分析和存储的用户界面。该系统使用Linux、Mac OS X和Windows主机从各种探测器类型收集列表模式数据、相关脉冲形状或流式数据。它能提取商业系统上未发现的脉冲特征，在很宽的动态范围内具有出色 的灵敏度和线性度。列表模式数据以FCS 3.0格式文件保存，而流式或相关波形数据则以定制格式文件保存以供后期处理。开放式、可重新配置的细胞测量采集系统紧凑、可扩展、灵活且模块化。可编程特征提取算法为新的和现有的应用带来了令人兴奋的可能性。最近商用数据捕获板的出现将使类似系统得以广泛应用。
