嗜水气单胞菌几丁质酶基因在大肠杆菌中的克隆与表达
Cloning and expression of a chitinase gene from Aeromonas hydrophila in Escherichia coli.
作者信息
Chen J P, Nagayama F, Chang M C
机构信息
Council of Agriculture, Department of Food and Agriculture, Taipei, Republic of China.
出版信息
Appl Environ Microbiol. 1991 Aug;57(8):2426-8. doi: 10.1128/aem.57.8.2426-2428.1991.
Abstract
An extracellular secreted chitinase gene from Aeromonas hydrophila was cloned in Escherichia coli, and the gene product was detected in the culture medium. Like the natural chitinase protein, the excreted chitinase had a molecular weight of approximately 85,000 and was subject to catabolite repression by glucose.
摘要
从嗜水气单胞菌中克隆出一个细胞外分泌型几丁质酶基因，并将其在大肠杆菌中进行克隆，且在培养基中检测到了该基因产物。与天然几丁质酶蛋白一样，分泌的几丁质酶分子量约为85,000，且受葡萄糖的分解代谢物阻遏作用影响。
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