豚鼠气单胞菌chiA基因的克隆与一级结构
Cloning and primary structure of the chiA gene from Aeromonas caviae.
作者信息
Sitrit Y, Vorgias C E, Chet I, Oppenheim A B
机构信息
Otto Warburg Center for Agricultural Biotechnology, Faculty of Agriculture, Hebrew University, Rehovot, Israel.
出版信息
J Bacteriol. 1995 Jul;177(14):4187-9. doi: 10.1128/jb.177.14.4187-4189.1995.
Abstract
The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.
摘要
豚鼠气单胞菌的chiA基因编码一种胞外几丁质酶，该酶由865个氨基酸组成，与粘质沙雷氏菌的几丁质酶A具有高度相似性。在大肠杆菌中表达产生了一种具有酶活性的蛋白质，推测在该酶跨细胞膜转运过程中其前导序列被去除。
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