• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Cloning and primary structure of the chiA gene from Aeromonas caviae.豚鼠气单胞菌chiA基因的克隆与一级结构
J Bacteriol. 1995 Jul;177(14):4187-9. doi: 10.1128/jb.177.14.4187-4189.1995.
2
Cloning, DNA sequence, and expression of Aeromonas caviae WS7b chitinase gene.
Mol Biotechnol. 2003 Jan;23(1):1-10. doi: 10.1385/MB:23:1:1.
3
Cloning of a cluster of chitinase genes from Aeromonas sp. No. 10S-24.从气单胞菌属10S-24菌株中克隆一组几丁质酶基因
Biochim Biophys Acta. 1996 Feb 7;1305(1-2):44-8. doi: 10.1016/0167-4781(95)00213-8.
4
Site-directed mutagenesis of Asp313, Glu315, and Asp391 residues in chitinase of Aeromonas caviae.豚鼠气单胞菌几丁质酶中Asp313、Glu315和Asp391残基的定点诱变
IUBMB Life. 1999 Aug;48(2):199-204. doi: 10.1080/713803487.
5
Expression and characterization of the recombinant gene encoding chitinase from Aeromonas caviae.豚鼠气单胞菌几丁质酶重组基因的表达与鉴定
Enzyme Microb Technol. 1997 Nov 15;21(7):472-8. doi: 10.1016/s0141-0229(96)00249-9.
6
The C-terminal module of Chi1 from Aeromonas caviae CB101 has a function in substrate binding and hydrolysis.豚鼠气单胞菌CB101的Chi1蛋白C端模块在底物结合和水解中发挥作用。
Proteins. 2003 Dec 1;53(4):908-16. doi: 10.1002/prot.10501.
7
Cloning and expression of a chitinase gene from Aeromonas hydrophila in Escherichia coli.嗜水气单胞菌几丁质酶基因在大肠杆菌中的克隆与表达
Appl Environ Microbiol. 1991 Aug;57(8):2426-8. doi: 10.1128/aem.57.8.2426-2428.1991.
8
Aeromonas caviae CB101 contains four chitinases encoded by a single gene chi1.豚鼠气单胞菌 CB101 含有一个基因 chi1 编码的四种几丁质酶。
Mol Biotechnol. 2010 Mar;44(3):213-20. doi: 10.1007/s12033-009-9227-z.
9
Expression of the chitinase III gene of Aeromonas sp. no. 10S-24 in Escherichia coli.嗜水气单胞菌10S - 24几丁质酶III基因在大肠杆菌中的表达
Biosci Biotechnol Biochem. 1996 Jul;60(7):1195-7. doi: 10.1271/bbb.60.1195.
10
Molecular cloning and purification of an endochitinase from Serratia marcescens (Nima).粘质沙雷氏菌（尼玛）内切几丁质酶的分子克隆与纯化
Mol Biotechnol. 2005 Oct;31(2):103-11. doi: 10.1385/MB:31:2:103.

引用本文的文献

1
Exploring the metabolic potential of to utilise the carbohydrate polymer chitin.探索利用碳水化合物聚合物几丁质的代谢潜力。
RSC Chem Biol. 2024 Dec 9;6(2):227-239. doi: 10.1039/d4cb00200h. eCollection 2025 Feb 5.
2
Chitin and Chitosan: Production and Application of Versatile Biomedical Nanomaterials.几丁质与壳聚糖：多功能生物医学纳米材料的生产与应用
Int J Adv Res (Indore). 2016 Mar;4(3):411-427. Epub 2016 Mar 1.
3
Cooperative degradation of chitin by extracellular and cell surface-expressed chitinases from Paenibacillus sp. strain FPU-7.来自短小芽孢杆菌 FPU-7 的细胞外和细胞表面表达的几丁质酶协同降解几丁质。
Appl Environ Microbiol. 2013 Dec;79(23):7482-90. doi: 10.1128/AEM.02483-13. Epub 2013 Sep 27.
4
Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli.紫色色杆菌几丁质酶在大肠杆菌中的表达和有效分泌。
BMC Biotechnol. 2013 Jun 1;13:46. doi: 10.1186/1472-6750-13-46.
5
Chitinases: An update.几丁质酶：最新进展
J Pharm Bioallied Sci. 2013 Jan;5(1):21-9. doi: 10.4103/0975-7406.106559.
6
Microdiversity of extracellular enzyme genes among sequenced prokaryotic genomes.原核生物基因组中细胞外酶基因的微观多样性。
ISME J. 2013 Jun;7(6):1187-99. doi: 10.1038/ismej.2012.176. Epub 2013 Jan 10.
7
Cloning, characterization and expression of the chitinase gene of Enterobacter sp. NRG4.肠杆菌 NRG4 几丁质酶基因的克隆、特性分析与表达。
Indian J Microbiol. 2008 Sep;48(3):358-64. doi: 10.1007/s12088-008-0044-z. Epub 2009 Mar 25.
8
Substrate binding modes and anomer selectivity of chitinase A from Vibrio harveyi.哈维氏弧菌几丁质酶A的底物结合模式和异头物选择性
J Chem Biol. 2009 Nov;2(4):191-202. doi: 10.1007/s12154-009-0021-y. Epub 2009 May 28.
9
Cloning, DNA sequence, and expression of Aeromonas caviae WS7b chitinase gene.
Mol Biotechnol. 2003 Jan;23(1):1-10. doi: 10.1385/MB:23:1:1.
10
Molecular analysis of the gene encoding a novel chitin-binding protease from Alteromonas sp. strain O-7 and its role in the chitinolytic system.对来自交替单胞菌属菌株O-7的一种新型几丁质结合蛋白酶编码基因的分子分析及其在几丁质分解系统中的作用。
J Bacteriol. 2002 Apr;184(7):1865-72. doi: 10.1128/JB.184.7.1865-1872.2002.

本文引用的文献

1
Isolation and characterization of genes encoding two chitinase enzymes from Serratia marcescens.从粘质沙雷氏菌中分离和鉴定编码两种几丁质酶的基因。
EMBO J. 1986 Mar;5(3):467-73. doi: 10.1002/j.1460-2075.1986.tb04235.x.
2
Cloning, sequence, and expression of a chitinase gene from a marine bacterium, Altermonas sp. strain O-7.来自海洋细菌交替单胞菌属O-7菌株的几丁质酶基因的克隆、测序及表达
J Bacteriol. 1993 Jan;175(1):176-81. doi: 10.1128/jb.175.1.176-181.1993.
3
Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacillus circulans WL-12 as essential residues for chitinase activity.鉴定环状芽孢杆菌WL-12几丁质酶A1中的谷氨酸204和天冬氨酸200为几丁质酶活性的必需残基。
J Biol Chem. 1993 Sep 5;268(25):18567-72.
4
Crystal structure of a bacterial chitinase at 2.3 A resolution.分辨率为2.3埃的细菌几丁质酶晶体结构。
Structure. 1994 Dec 15;2(12):1169-80. doi: 10.1016/s0969-2126(94)00119-7.
5
A bacteriophage lambda vector for cloning with BamHI and Sau3A.一种用于用BamHI和Sau3A进行克隆的λ噬菌体载体。
Gene. 1982 Dec;20(3):317-22. doi: 10.1016/0378-1119(82)90200-1.
6
Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology.嗜碱芽孢杆菌N-4菌株中两个纤维素酶基因的核苷酸序列及其高度同源性。
J Bacteriol. 1986 Nov;168(2):479-85. doi: 10.1128/jb.168.2.479-485.1986.
7
Structure of the gene encoding chitinase D of Bacillus circulans WL-12 and possible homology of the enzyme to other prokaryotic chitinases and class III plant chitinases.环状芽孢杆菌WL-12几丁质酶D编码基因的结构以及该酶与其他原核几丁质酶和Ⅲ类植物几丁质酶可能的同源性。
J Bacteriol. 1992 Jan;174(2):408-14. doi: 10.1128/jb.174.2.408-414.1992.

豚鼠气单胞菌chiA基因的克隆与一级结构

Cloning and primary structure of the chiA gene from Aeromonas caviae.

作者信息

Sitrit Y, Vorgias C E, Chet I, Oppenheim A B

机构信息

Otto Warburg Center for Agricultural Biotechnology, Faculty of Agriculture, Hebrew University, Rehovot, Israel.

出版信息

J Bacteriol. 1995 Jul;177(14):4187-9. doi: 10.1128/jb.177.14.4187-4189.1995.

DOI:10.1128/jb.177.14.4187-4189.1995
PMID:7608101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177160/
Abstract

The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.

摘要

豚鼠气单胞菌的chiA基因编码一种胞外几丁质酶，该酶由865个氨基酸组成，与粘质沙雷氏菌的几丁质酶A具有高度相似性。在大肠杆菌中表达产生了一种具有酶活性的蛋白质，推测在该酶跨细胞膜转运过程中其前导序列被去除。