Yang Seung-Jo, Choo Yoe-Jin, Cho Jang-Cheon
Division of Biology and Ocean Sciences, Inha University, Yonghyun-Dong, Incheon 402-751, Republic of Korea.
Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1679-1684. doi: 10.1099/ijs.0.65060-0.
A Gram-negative, yellow-coloured, non-motile, chemoheterotrophic, strictly aerobic, rod-shaped bacterium, designated IMCC1616(T), was isolated from the marine polychaete Periserrula leucophryna inhabiting tidal flat sediment of the Yellow Sea, and characterized by a polyphasic approach. The temperature, pH and NaCl ranges for growth were 3-37 degrees C, pH 5.0-11.0 and 0.5-7.5 %. Based on 16S rRNA gene sequence similarity analyses, the strain was most closely related to members of the genera Lutibacter (90.7 %), Tenacibaculum (89.2-90.4 %) and Polaribacter (88.4-90.2 %). Phylogenetic analysis using three treeing algorithms based on 16S rRNA gene sequences indicated that the strain formed a distinct lineage within the family Flavobacteriaceae. The DNA G+C content of the strain was 40.1 mol% and the predominant cellular fatty acids were iso-C(15 : 0) (16.5 %), anteiso-C(15 : 0) (10.9 %), iso-C(17 : 0) 3-OH (8.8 %) and iso-C(17 : 1) omega 9c (8.2 %). The DNA G+C content, large amount of iso-C(17 : 1) omega 9c and several phenotypic characteristics, including growth temperature and catalase activity, differentiated the strain from other related genera in the family. Therefore, from the taxonomic evidence collected in this study, it is proposed that strain IMCC1616(T) represents a new genus and species named Lutimonas vermicola gen. nov., sp. nov. The type strain of Lutimonas vermicola is strain IMCC1616(T) (=KCCM 42379(T) =NBRC 102041(T)).
从黄海潮间带沉积物中栖息的海洋多毛类动物白须围沙蚕（Periserrula leucophryna）中分离出一株革兰氏阴性、黄色、无运动性、化能异养、严格需氧的杆状细菌，命名为IMCC1616(T)，并采用多相分类法对其进行了特征描述。其生长的温度范围为3-37℃，pH范围为5.0-11.0，NaCl浓度范围为0.5-7.5%。基于16S rRNA基因序列相似性分析，该菌株与嗜盐杆菌属（Lutibacter）成员（相似度90.7%）、黄杆菌属（Tenacibaculum）成员（相似度89.2-90.4%）和极地杆菌属（Polaribacter）成员（相似度88.4-90.2%）关系最为密切。基于16S rRNA基因序列使用三种建树算法进行的系统发育分析表明，该菌株在黄杆菌科（Flavobacteriaceae）内形成了一个独特的分支。该菌株的DNA G+C含量为40.1 mol%，主要细胞脂肪酸为异-C(15 : 0)（16.5%）、anteiso-C(15 : 0)（10.9%）、异-C(17 : 0) 3-OH（8.8%）和异-C(17 : 1) ω9c（8.2%）。DNA G+C含量、大量的异-C(17 : 1) ω9c以及包括生长温度和过氧化氢酶活性在内的几个表型特征，使该菌株与该科其他相关属区分开来。因此，根据本研究收集的分类学证据，建议菌株IMCC1616(T)代表一个新的属和种，命名为嗜泥栖盐单胞菌（Lutimonas vermicola），属名新属，种名新种。嗜泥栖盐单胞菌的模式菌株为IMCC1616(T)（=KCCM 42379(T) =NBRC 102041(T)）。
