Lee Chang Soo, Kim Kwang Kyu, Aslam Zubair, Lee Sung-Taik
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong, Yuseong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1775-1779. doi: 10.1099/ijs.0.65086-0.
A novel thiosulfate-oxidizing bacterium, designated strain LCS2(T), was isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. The strain was found to comprise Gram-negative, non-motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. The strain contained Q-8 as the major ubiquinone and 17 : 1 iso omega 9c, 15 : 0 iso and 17 : 0 iso as the major fatty acids. The G+C content of the genomic DNA was 64.6 mol%. The 16S rRNA gene sequence of strain LCS2(T) was found to be most similar to that of Rhodanobacter fulvus IAM 15025(T) (97.4 % similarity). The results of DNA-DNA hybridization and phenotypic analysis showed that strain LCS2(T) can be distinguished from all known Rhodanobacter species and therefore represents a novel species of the genus, for which the name Rhodanobacter thiooxydans sp. nov. is proposed. The type strain is LCS2(T) (=DSM 18863(T) =KCTC 12771(T)).
从自养反硝化过程中使用的硫颗粒上的生物膜中分离出一株新型硫代硫酸盐氧化细菌，命名为菌株LCS2(T)。该菌株为革兰氏阴性、不运动、不产芽孢的杆菌，在R2A琼脂上形成黄色色素菌落。该菌株以Q-8作为主要泛醌，以17 : 1异ω9c、15 : 0异和17 : 0异作为主要脂肪酸。基因组DNA的G+C含量为64.6 mol%。发现菌株LCS2(T)的16S rRNA基因序列与黄红红杆菌IAM 15025(T)的序列最相似(相似性为97.4%)。DNA-DNA杂交和表型分析结果表明，菌株LCS2(T)可与所有已知的红红杆菌属物种区分开来，因此代表该属的一个新物种，为此提出了硫氧化红红杆菌新种的名称。模式菌株为LCS2(T)(=DSM 18863(T)=KCTC 12771(T))。
