Dahal Ram Hari, Chaudhary Dhiraj Kumar, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Aug;68(8):2580-2586. doi: 10.1099/ijsem.0.002881. Epub 2018 Jun 28.
A bacterial isolate, designated G-5-5, was isolated from forest soil at Kyonggi University. Strain G-5-5 was acid-tolerant and alkali-tolerant. Cells were strictly aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, rod-shaped, and yellow-coloured. Strain G-5-5 hydrolysed DNA and tyrosine; assimilated d-glucose, maltose, N-acetyl-glucosamine and l-fucose; and tolerated only 0.5 % NaCl (w/v). Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain G-5-5 formed a lineage within the family Rhodanobacteraceae and that it grouped with but was distinct from various members of the genus Rhodanobacter. The closest member was Rhodanobacter umsongensis GR24-2 (97.8 % sequence similarity). The sole respiratory quinone was Q-8. The major polar lipids of strain G-5-5 were phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major cellular fatty acids were summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C15 : 0, iso-C17 : 0, iso-C16 : 0 and anteiso-C15 : 0. The DNA G+C content of strain G-5-5 was 64.1 mol%. DNA-DNA hybridization relatedness between strain G-5-5 and other close members of the genus Rhodanobacter ranged from 19 % to 45 %. On the basis of the polyphasic characterization and phylogenetic analyses, strain G-5-5 represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter hydrolyticus sp. nov. is proposed. The type strain is G-5-5 (=KEMB 9005-533=KACC 19113=NBRC 112685).
从京畿大学的森林土壤中分离出一株细菌，命名为G-5-5。G-5-5菌株耐酸且耐碱。细胞严格需氧，革兰氏染色阴性，过氧化氢酶和氧化酶阳性，不运动，不产芽孢，杆状，黄色。G-5-5菌株能水解DNA和酪氨酸；同化d-葡萄糖、麦芽糖、N-乙酰葡糖胺和L-岩藻糖；仅能耐受0.5%（w/v）的NaCl。基于其16S rRNA基因序列的系统发育分析表明，G-5-5菌株在红杆菌科内形成一个分支，并且它与红杆菌属的各个成员聚在一起，但又有所不同。最接近的成员是翁松红杆菌GR24-2（序列相似性为97.8%）。唯一的呼吸醌是Q-8。G-5-5菌株的主要极性脂类是磷脂酰乙醇胺、磷脂酰-N-甲基乙醇胺、磷脂酰甘油和二磷脂酰甘油。主要的细胞脂肪酸是总和特征9（异-C17 : 1ω9c和/或C16 : 0 10-甲基）、异-C15 : 0、异-C17 : 0、异-C16 : 0和anteiso-C15 : 0。G-5-5菌株的DNA G+C含量为64.1 mol%。G-5-5菌株与红杆菌属其他近缘成员之间的DNA-DNA杂交相关性在19%至45%之间。基于多相特征描述和系统发育分析，G-5-5菌株代表红杆菌属的一个新物种，为此提出名为水解红杆菌（Rhodanobacter hydrolyticus）的新物种。模式菌株是G-5-5（=KEMB 9005-533=KACC 19113=NBRC 112685）。
