Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 443-760, Republic of Korea.
Int J Syst Evol Microbiol. 2017 May;67(5):1185-1190. doi: 10.1099/ijsem.0.001786. Epub 2017 May 30.
A novel acid-tolerant and alkalitolerant gammaproteobacterium designated strain RS22T was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22T was able to assimilate lactic acid, l-proline and 3-hydroxybenzoic acid; it tolerated 4 % (w/v) NaCl, fermented glucose and was able to grow at pH 11.0. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22T formed a lineage within the class Gammaproteobacteria of the phylum Proteobacteria that was distinct from various species of the genus Rhodanobacter, including Rhodanobacter denitrificans 2APBS1T (97.7 % sequence similarity), Rhodanobacter thiooxydans LCS2T (97.5 %), Rhodanobacter terrae GP18-1T (97.5 %) and Rhodanobacter soli DCY45T (97.2 %). The predominant respiratory quinone was Q-8. The major polar lipids of strain RS22T were phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids were summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C15 : 0, iso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and C16 : 0. The DNA G+C content of strain RS22T was 63.2 mol%. DNA-DNA hybridization between strain RS22T and other closest members of the genus Rhodanobacterrevealed relatedness values from 28 to 51 %. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain RS22T represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter humi sp. nov. is proposed. The type strain is RS22T (=KEMB 9005-480T=KACC 19048T=NBRC 112473T).
一株耐酸耐碱的γ-变形菌新种 RS22T 的分离与鉴定
从京畿大学森林土壤中分离到一株耐酸耐碱的γ-变形菌，命名为 RS22T 株。该菌株细胞呈革兰氏阴性，好氧，不产芽孢，不运动，无鞭毛，直杆状，黄色，能利用乳酸、L-脯氨酸和 3-羟基苯甲酸，耐受 4%（质量分数）NaCl，发酵葡萄糖，最适生长 pH 为 11.0。该菌株的 16S rRNA 基因序列与 Rhodanobacter 属中各个种的相似性均低于 97.7%，而与 Rhodanobacter denitrificans 2APBS1T（97.7%）、Rhodanobacter thiooxydans LCS2T（97.5%）、Rhodanobacter terrae GP18-1T（97.5%）和 Rhodanobacter soli DCY45T（97.2%）等种的相似性均低于 97.5%，因此，该菌株在系统发育上形成了γ-变形菌目中一个与 Rhodanobacter 属中各个种均不同的分支。该菌株的醌类为 Q-8，主要极性脂为磷脂酰乙醇胺、磷酯酰-N-甲基乙醇胺、双磷脂酰甘油和磷脂酰甘油，主要脂肪酸为 iso-C15：0、iso-C17：0、iso-C16：0、anteiso-C15：0、iso-C17：1ω9c 和/或 C16：0 10-methyl、C16：0。该菌株的 G+C 含量为 63.2 mol%。基于表型、基因型、化学分类和系统发育分析，将菌株 RS22T 鉴定为 Rhodanobacter 属的一个新种，命名为 Rhodanobacter humi sp. nov.，模式菌株为 RS22T（=KEMB 9005-480T=KACC 19048T=NBRC 112473T）。
