Chauhan Lalit K S, Kumar Munish, Paul Bhola N, Goel Sudhir K, Gupta Shrawan K
Cell Biology Division, Industrial Toxicology Research Centre, Lucknow, India.
Environ Mol Mutagen. 2007 Oct;48(8):636-43. doi: 10.1002/em.20330.
The cytogenetic effects of deltamethrin (DEL) and/or isoproturon (ISO) were examined in human lymphocytes and mouse bone marrow cells. Peripheral lymphocytes were exposed to DEL (2.5, 5, 10, or 20 microM), ISO (25, 50, 100, or 200 microM), or DEL + ISO (2.5 + 25, 5 + 50, 10 + 100, or 20 + 200 microM) and cytogenic effects were evaluated via chromosomal aberrations (CA) and the cytokinesis-block micronucleus assay (CBMN). Mice were orally gavaged to single dose of DEL (6.6 mg/kg), ISO (670 mg/kg), or DEL+ISO (6.6 + 670 mg/kg) for 24 hr or to DEL (3.3 mg/kg/day), ISO (330 mg/kg/day), or DEL + ISO (3.3 + 330 mg/kg/day) for 30 days and analyzed for CA. DEL induced a significant frequency of CA at 10 microM whereas ISO (25-100 microM) alone, or in combination with DEL, did not show any significant effect. Micronucleus (MN) induction was observed to be concentration-dependent though significant frequencies were observed at 5 microM DEL, 100 microM ISO, or 5 + 50 microM DEL + ISO. In mice, DEL inhibited the mitotic index (MI) significantly (P < 0.001) at 24 hr while ISO alone, or in combination with DEL, did not cause any statistically significant effect. Following a 24 hr exposure, DEL and ISO alone induced significant (P < 0.01) frequencies of CA, whereas DEL + ISO in combination did not. Furthermore, 30 days exposure of ISO significantly inhibited the MI (P < 0.02 or < 0.01) and induced CA while DEL alone, or in combination with ISO, resulted in no significant effect on CA or the MI. The present findings indicate that the in vitro and in vivo exposure of a commercial formulation of DEL can cause genotoxic effects in mammals. However, the coexposure of DEL and ISO did not show additive effects, but instead demonstrated somewhat reduced genotoxicity.
在人淋巴细胞和小鼠骨髓细胞中检测了溴氰菊酯（DEL）和/或异丙隆（ISO）的细胞遗传学效应。将外周血淋巴细胞暴露于DEL（2.5、5、10或20微摩尔）、ISO（25、50、100或200微摩尔）或DEL + ISO（2.5 + 25、5 + 50、10 + 100或20 + 200微摩尔），并通过染色体畸变（CA）和胞质分裂阻滞微核试验（CBMN）评估细胞遗传学效应。给小鼠口服单剂量的DEL（6.6毫克/千克）、ISO（670毫克/千克）或DEL + ISO（6.6 + 670毫克/千克）24小时，或口服DEL（3.3毫克/千克/天）、ISO（330毫克/千克/天）或DEL + ISO（3.3 + 330毫克/千克/天）30天，然后分析CA。DEL在10微摩尔时诱导出显著频率的CA，而单独的ISO（25 - 100微摩尔）或与DEL联合使用时，未显示出任何显著影响。观察到微核（MN）诱导呈浓度依赖性，尽管在5微摩尔DEL、100微摩尔ISO或5 + 50微摩尔DEL + ISO时观察到显著频率。在小鼠中，DEL在24小时时显著抑制有丝分裂指数（MI）（P < 0.001），而单独的ISO或与DEL联合使用时，未产生任何统计学上显著的影响。暴露24小时后，单独的DEL和ISO诱导出显著（P < 0.01）频率的CA，而DEL + ISO联合使用时则没有。此外，暴露30天的ISO显著抑制MI（P < 0.02或< 0.01）并诱导CA，而单独的DEL或与ISO联合使用时，对CA或MI均未产生显著影响。目前的研究结果表明，商业配方的DEL在体外和体内暴露均可导致哺乳动物产生遗传毒性效应。然而，DEL和ISO的共同暴露未显示出相加效应，反而表现出一定程度的遗传毒性降低。
