Xiang Guang-da, Sun Hui-ling, Zhao Lin-shuang, Hou Jie, Yue Ling, Xu Lin
Department of Endocrinology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China.
Zhonghua Yi Xue Za Zhi. 2007 May 15;87(18):1234-7.
To investigate the relationship between the plasma osteoprotegerin (OPG) level and endothelium-dependent arterial dilation in type 1 diabetic patients.
Sandwich ELISA method was used to detect the plasma OPG levels of 22 newly diagnosed type 1 diabetic patients before and 6 months after treatment and of 28 healthy subjects. All patients were then given insulin therapy for 6 months. High resolution ultrasound was used to measure the brachial artery diameter at rest, after reactive hyperemia and after sublingual administration of glyceryltrinitrate (GTN).
The plasma OPG level of the patients before treatment was 3.09 ng/L +/- 0.70 ng/L, significantly higher than that of the healthy controls (2.07 ng/L +/- 0.75 ng/L, P < 0.001). After 6 months treatment, the OPG level of the patients decreased to 2.58 ng/L +/- 0.59 ng/L, significantly lower than that before treatment (P < 0.001). The flow-mediated endothelium-dependent arterial dilation in the patients before treatment was 3.35% +/- 0.67%, significantly lower than that of the healthy controls (5.17% +/- 0.83%, P < 0.001), and was increased to 4.27% +/- 0.63% after 6 months treatment, significantly higher than that before (P < 0.001). Multivariate analysis showed that OPG level was significantly associated with endothelium-dependent arterial dilation, fasting blood glucose (FBG), hemoglobin A1c (HbA1c), and ultra-sensitive C-reactive protein (CRP) at baseline (all P < 0.01). The absolute change in OPG level was significantly correlation with the changes in endothelium-dependent arterial dilation, FBG, HbA1c, and CRP in the diabetic patients during the course of treatment (all P < 0.01).
Plasma OPG level is elevated in newly diagnosed diabetic patients, and the plasma OPG level is significantly associated with endothelial function.
探讨1型糖尿病患者血浆骨保护素（OPG）水平与内皮依赖性动脉扩张之间的关系。
采用夹心酶联免疫吸附测定法（ELISA）检测22例新诊断的1型糖尿病患者治疗前及治疗6个月后的血浆OPG水平，以及28例健康受试者的血浆OPG水平。所有患者均接受6个月的胰岛素治疗。使用高分辨率超声测量静息时、反应性充血后及舌下含服硝酸甘油（GTN）后的肱动脉内径。
治疗前患者血浆OPG水平为3.09 ng/L±0.70 ng/L，显著高于健康对照组（2.07 ng/L±0.75 ng/L，P<0.001）。治疗6个月后，患者的OPG水平降至2.58 ng/L±0.59 ng/L，显著低于治疗前（P<0.001）。治疗前患者的血流介导的内皮依赖性动脉扩张为3.35%±0.67%，显著低于健康对照组（5.17%±0.83%，P<0.001），治疗6个月后升高至4.27%±0.63%，显著高于治疗前（P<0.001）。多变量分析显示，基线时OPG水平与内皮依赖性动脉扩张、空腹血糖（FBG）、糖化血红蛋白（HbA1c）及超敏C反应蛋白（CRP）显著相关（均P<0.01）。糖尿病患者治疗过程中OPG水平的绝对变化与内皮依赖性动脉扩张、FBG、HbA1c及CRP的变化显著相关（均P<0.01）。
新诊断的糖尿病患者血浆OPG水平升高，且血浆OPG水平与内皮功能显著相关。
