Ueda T, Watanabe A, Ishihara K, Nakabayashi N
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
J Biomater Sci Polym Ed. 1991;3(2):185-94. doi: 10.1163/156856291x00278.
The effects of phospholipid adsorption onto the polymer surface during adsorption of plasma proteins were investigated. When a polymer with the phosphorylcholine moiety, 2-methacryloyloxyethyl phosphorylcholine (MPC) co-polymer, was treated with dipalmitoylphosphatidylcholine (DPPC) liposome solution, an organized adsorption layer of DPPC was formed on the MPC co-polymer surface, which was confirmed by differential scanning calorimetric analysis and X-ray photoelectron spectroscopy. On the other hand, an organized layer of DPPC on poly(n-butyl methacrylate) and poly(2-hydroxyethyl methacrylate) could not be found. The amount of albumin adsorbed on the polymer surfaces was decreased by pretreatment of the surface with DPPC liposome solution in every polymer case. The smallest amount of adsorbed proteins was found on the MPC co-polymer. Protein adsorption on the surface of MPC co-polymers from the plasma was also small. The difference in protein adsorption on the polymers probably reflects the difference in the orientation of the phospholipid molecules which cover the polymer surface.
研究了血浆蛋白吸附过程中磷脂在聚合物表面的吸附作用。当含磷酰胆碱部分的聚合物，即2-甲基丙烯酰氧乙基磷酰胆碱（MPC）共聚物，用二棕榈酰磷脂酰胆碱（DPPC）脂质体溶液处理时，在MPC共聚物表面形成了有组织的DPPC吸附层，这通过差示扫描量热分析和X射线光电子能谱得以证实。另一方面，在聚甲基丙烯酸正丁酯和聚甲基丙烯酸2-羟乙酯上未发现有组织的DPPC层。在每种聚合物情况下，用DPPC脂质体溶液对聚合物表面进行预处理后，聚合物表面吸附的白蛋白量均减少。在MPC共聚物上发现的吸附蛋白量最少。从血浆中吸附到MPC共聚物表面的蛋白质也很少。聚合物上蛋白质吸附的差异可能反映了覆盖聚合物表面的磷脂分子取向的差异。
