Kojima M, Ishihara K, Watanabe A, Nakabayashi N
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
Biomaterials. 1991 Mar;12(2):121-4. doi: 10.1016/0142-9612(91)90189-h.
Random and block copolymers containing a phospholipid polar group in their side chain were synthesized by the copolymerization between 2-methacryloyloxyethyl phosphorylcholine and styrene. These copolymers showed amphiphilic character, especially poly(methacryloyloxyethyl phosphorylcholine-block-styrene) formed stable polymer micelles in water. The interaction between natural phospholipid, dipalmitoylphosphatidylcholine and methacryloyloxyethyl phosphorylcholine copolymers was investigated. The amount absorbed of dipalmitoylphosphatidylcholine from its liposomal solution on to the poly(methacryloyloxyethyl phosphorylcholine-co-styrene) surface increased with increase of methacryloyloxyethyl phosphorylcholine composition. Moreover, when poly(methacryloyloxyethyl phosphorylcholine-block-styrene) was added to dipalmitoylphosphatidylcholine solution, organization of dipalmitoylphosphatidylcholine molecules and stabilization of bilayer structure of dipalmitoylphosphatidylcholine liposome were found. This means that methacryloyloxyethyl phosphorylcholine moieties in the copolymer have a strong affinity to dipalmitoylphosphatidylcholine molecules. The blood compatibility of methacryloyloxyethyl phosphorylcholine copolymers was also investigated with particular attention to the aggregation ability of platelets after contacting methacryloyloxyethyl phosphorylcholine copolymers; this ability decreased when platelets were put in contact with polymers without a methacryloyloxyethyl phosphorylcholine moiety. On the other hand, aggregation ability remained at almost the same level to that of original platelets after contact with methacryloyloxyethyl phosphorylcholine copolymers. From these findings, we concluded that methacryloyloxyethyl phosphorylcholine copolymers show excellent blood compatibility due to adsorption of lipids from plasma and the formation of an organized adsorption layer of lipids on the surface of the methacryloyloxyethyl phosphorylcholine copolymers.
通过2-甲基丙烯酰氧乙基磷酰胆碱与苯乙烯的共聚反应，合成了侧链含有磷脂极性基团的无规共聚物和嵌段共聚物。这些共聚物表现出两亲性，尤其是聚（甲基丙烯酰氧乙基磷酰胆碱-嵌段-苯乙烯）在水中形成了稳定的聚合物胶束。研究了天然磷脂二棕榈酰磷脂酰胆碱与甲基丙烯酰氧乙基磷酰胆碱共聚物之间的相互作用。二棕榈酰磷脂酰胆碱从其脂质体溶液吸附到聚（甲基丙烯酰氧乙基磷酰胆碱-共-苯乙烯）表面的量随着甲基丙烯酰氧乙基磷酰胆碱组成的增加而增加。此外，当将聚（甲基丙烯酰氧乙基磷酰胆碱-嵌段-苯乙烯）添加到二棕榈酰磷脂酰胆碱溶液中时，发现二棕榈酰磷脂酰胆碱分子的有序排列以及二棕榈酰磷脂酰胆碱脂质体双层结构的稳定化。这意味着共聚物中的甲基丙烯酰氧乙基磷酰胆碱部分对二棕榈酰磷脂酰胆碱分子具有很强的亲和力。还研究了甲基丙烯酰氧乙基磷酰胆碱共聚物的血液相容性，特别关注血小板与甲基丙烯酰氧乙基磷酰胆碱共聚物接触后的聚集能力；当血小板与不含甲基丙烯酰氧乙基磷酰胆碱部分的聚合物接触时，这种能力降低。另一方面，血小板与甲基丙烯酰氧乙基磷酰胆碱共聚物接触后，聚集能力与原始血小板的聚集能力几乎保持在同一水平。从这些发现中，我们得出结论，甲基丙烯酰氧乙基磷酰胆碱共聚物由于从血浆中吸附脂质并在甲基丙烯酰氧乙基磷酰胆碱共聚物表面形成有组织的脂质吸附层而表现出优异的血液相容性。
