Bocci V, Luzzi E, Corradeschi F, Paulesu L, Di Stefano A
Institute of General Physiology, Faculty of Pharmacy, University of Siena, Italy.
Lymphokine Cytokine Res. 1993 Apr;12(2):121-6.
Ozonization of blood, normally carried out with citrated blood, may be fine for the autohemotherapy of ischemic diseases but it may be at a loss when employed in viral diseases or in immunodeficiencies. We have shown that heparin, used as an anticoagulant, with the addition of 5 mM CaCl2 favors production of cytokines by leukocytes with only a modest increase in hemolysis. High plasmatic levels of glucose, glutathione, and ascorbic acid decrease cytokine's yield because these compounds act as antioxidants and quench the inducing activity of ozone. Autohemotherapy with heparinized and Ca(2+)-supplemented blood has not revealed any side effects in volunteers.
血液的臭氧处理通常使用枸橼酸盐血进行，这对于缺血性疾病的自血疗法可能是合适的，但在用于病毒性疾病或免疫缺陷时可能效果不佳。我们已经表明，用作抗凝剂的肝素，添加5 mM氯化钙后，有利于白细胞产生细胞因子，同时溶血仅适度增加。高血浆水平的葡萄糖、谷胱甘肽和抗坏血酸会降低细胞因子的产量，因为这些化合物作为抗氧化剂，会淬灭臭氧的诱导活性。用肝素化且补充了钙的血液进行自血疗法在志愿者中未发现任何副作用。
