Matsumoto K
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Clin Nephrol. 1991 Dec;36(6):267-73.
Monocytes from 30 patients with glomerulonephritis (GN) were stimulated with soluble immune complexes (IC). In order to neutralize the effect of prostaglandins, some cultures were incubated in the presence of indomethacin. Interleukin-1 (IL-1) activity was assessed by the comitogenic activity of the crude monocyte supernatants on phytohemagglutinin (PHA)-stimulated murine thymocytes. Our results demonstrate that, upon stimulation with the soluble IC monocytes from GN patients possess an enhanced capacity to produce IL-1, and the levels of IL-1 correlate with disease activity only in one case of acute poststreptococcal GN (AGN), not in all other patients. This enhanced production of IL-1 may contribute to the disordered immunoregulation in GN.
用可溶性免疫复合物（IC）刺激30例肾小球肾炎（GN）患者的单核细胞。为了中和前列腺素的作用，一些培养物在吲哚美辛存在的情况下孵育。通过粗单核细胞上清液对植物血凝素（PHA）刺激的小鼠胸腺细胞的促有丝分裂活性来评估白细胞介素-1（IL-1）活性。我们的结果表明，GN患者的单核细胞在用可溶性IC刺激后产生IL-1的能力增强，并且IL-1水平仅在1例急性链球菌感染后肾小球肾炎（AGN）中与疾病活动相关，在所有其他患者中并非如此。IL-1的这种产生增加可能导致GN中免疫调节紊乱。
