Matsumoto K
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Int Urol Nephrol. 1992;24(3):319-26. doi: 10.1007/BF02549542.
We have investigated the effect of immune complexes (IC) derived from human renal tubular epithelial (RTE) antigen on the release of interleukin-1 (IL-1) in monocyte cultures from patients with glomerulonephritis (GN). When peripheral blood monocytes (PBM) were activated by IC, substantial amounts of IL-1 could be detected in the supernatants as measured by mouse thymocyte assay. The IC-induced IL-1 activity was significantly higher in patients with GN than in normal controls. To avoid the effect of prostaglandins on the IL-1 assay, we cultured PBM with addition of indomethacin and assayed IL-1 activity in the culture supernatants. This cyclooxygenase inhibitor augmented IC-induced IL-1 production. The results suggest that IC are involved in stimulating IL-1 production by PBM and thus play a role in the immune response in GN.
我们研究了源自人肾小管上皮（RTE）抗原的免疫复合物（IC）对肾小球肾炎（GN）患者单核细胞培养物中白细胞介素-1（IL-1）释放的影响。当外周血单核细胞（PBM）被IC激活时，通过小鼠胸腺细胞测定法可在上清液中检测到大量的IL-1。GN患者中IC诱导的IL-1活性显著高于正常对照。为避免前列腺素对IL-1测定的影响，我们在培养PBM时添加了吲哚美辛，并测定培养上清液中的IL-1活性。这种环氧化酶抑制剂增强了IC诱导的IL-1产生。结果表明，IC参与刺激PBM产生IL-1，从而在GN的免疫反应中发挥作用。
