磺基喹喔啉二酰基甘油对原核生物DNA聚合酶I活性及大肠杆菌细胞生长的抑制作用。

Inhibitory effect of sulfoquinovosyl diacylglycerol on prokaryotic DNA polymerase I activity and cell growth of Escherichia coli.

作者信息

Furukawa Tomoyuki, Nishida Masayuki, Hada Takahiko, Kuramochi Kouji, Sugawara Fumio, Kobayashi Susumu, Iijima Hiroshi, Shimada Hiroaki, Yoshida Hiromi, Mizushina Yoshiyuki

机构信息

Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, Noda, Chiba, JAPAN.

出版信息

J Oleo Sci. 2006;56(1):43-7. doi: 10.5650/jos.56.43.

DOI:10.5650/jos.56.43

PMID:17693698

Abstract

We isolated the glycolipids fraction from spinach (Spinacia oleracea L.) and found that the fraction inhibited the activities of prokaryotic DNA polymerase I from Escherichia coli (E. coli) and cell growth of E. coli. The fraction contained mainly three glycolipids, monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG) and sulfoquinovosyl diacylglycerol (SQDG), and purified SQDG inhibited these activities, however, purified MGDG and DGDG had no influence. In the tested strains of E. coli, SQDG inhibited the cell proliferation of the JM109 strain. It could be considered that a SQDG-containing thylakoid membrane in plant chloroplasts might have anti-bacterial activity.

摘要

我们从菠菜（Spinacia oleracea L.）中分离出糖脂组分，发现该组分可抑制大肠杆菌（E. coli）原核DNA聚合酶I的活性以及大肠杆菌的细胞生长。该组分主要包含三种糖脂，即单半乳糖基二酰基甘油（MGDG）、双半乳糖基二酰基甘油（DGDG）和磺基喹喔啉基二酰基甘油（SQDG），纯化后的SQDG可抑制这些活性，然而，纯化后的MGDG和DGDG则无影响。在测试的大肠杆菌菌株中，SQDG抑制JM109菌株的细胞增殖。可以认为，植物叶绿体中含SQDG的类囊体膜可能具有抗菌活性。