Johnston J B, Murray K, Cain R B
Antonie Van Leeuwenhoek. 1975;41(4):493-511. doi: 10.1007/BF02565092.
The reaction of 2,4-dinitroanilinomaleimide with sulphite which has been claimed as the basis of a suitable colorimetric assay for the anion was carefully re-examined. The sulphite-imide addition product provides a suitable and specific qualitative test for sulphite after separation by paper chromatography but the method as previously used is probably measuring the hydrolysis of the imide to 2,4-dinitroanilinomaleamic acid and cannot be used for sulphite determination either colorimetrically or in kinetic assays. A new colorimetric method for the determination of sulphite based on its reaction with Ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid) is described and compared for sensitivity with the p-rosaniline-HCHO method. Both methods were used to show the formation of sulphite as the initial product of arylsulphonate metabolism by bacteria. The failure to find sulphite in similar cultures of a third organism was attributed to the very high activities of sulphite oxidase found in extracts. The Ellman reagent was examined as the basis of an indicator medium for the detection of sulphite-excreting colonies.
2,4-二硝基苯胺马来酰亚胺与亚硫酸盐的反应（该反应被认为是一种适用于该阴离子的比色测定法的基础）被仔细地重新研究。亚硫酸盐-酰亚胺加成产物在通过纸色谱法分离后为亚硫酸盐提供了一种合适且特异的定性测试，但先前使用的方法可能是在测量酰亚胺水解为2,4-二硝基苯胺马来酰胺酸，因此无论是比色法还是动力学分析都不能用于亚硫酸盐的测定。本文描述了一种基于亚硫酸盐与埃尔曼试剂5,5'-二硫代双(2-硝基苯甲酸)反应的测定亚硫酸盐的新比色法，并将其灵敏度与对品红-甲醛法进行了比较。两种方法都用于表明亚硫酸盐是细菌代谢芳基磺酸盐的初始产物。在第三种生物体的类似培养物中未能检测到亚硫酸盐，这归因于提取物中发现的亚硫酸盐氧化酶的活性非常高。对埃尔曼试剂作为检测亚硫酸盐分泌菌落的指示培养基的基础进行了研究。
