Momohara Shigeki, Okada Naoyuki, Ikari Katsunori, Mizuno Shoji, Okamoto Hiroshi
Institute of Rheumatology, Tokyo Women's Medical University, 10-22 Kawada, Shinjuku-ku, Tokyo, 162-0054, Japan.
Mod Rheumatol. 2007;17(4):301-5. doi: 10.1007/s10165-007-0594-7. Epub 2007 Aug 20.
Biochemical factors play an important role in osteoarthritis (OA) pathogenesis. The purpose of this study is to clarify whether the dermatan sulfate (DS) levels in the synovial fluid of patients with knee OA are related to residual cartilage. Synovial fluid was obtained from 51 OA patients. Knee radiographs were evaluated with the Kellgren-Lawrence (K/L) grading scale. The levels of the following disaccharides were measured by high-performance liquid chromatography (HPLC): DS (DSDeltaDi4S), chondroitin 6-sulfate (CSDeltaDi6S), and chondroitin 4-sulfate (CSDeltaDi4S). The concentration of cartilage oligomeric matrix protein (COMP) was measured by a sandwich ELISA. The levels of DSDeltaDi4S in Grades 0 and I OA were significantly higher than levels in Grade II (P = 0.0458), Grade III (P < 0.0001) and Grade IV (P < 0.0001), and we found strong relationships between the levels of DSDeltaDi4S and those of CSDeltaDi6S (P < 0.0001, r = 0.705), CSDeltaDi4S (P < 0.0001, r = 0.750), and COMP (P < 0.0001, r = 0.699). We conclude that the presence of DSDeltaDi4S reflects proteoglycan metabolism in the residual articular cartilage of OA patients. This suggests that metabolism of the small leucine-rich repeat proteoglycans decorin and biglycan, which contain chains of DSDeltaDi4S, is similar to that of aggrecan.
生化因素在骨关节炎（OA）发病机制中起重要作用。本研究旨在阐明膝骨关节炎患者滑液中硫酸皮肤素（DS）水平与残余软骨是否相关。从51例OA患者获取滑液。采用Kellgren-Lawrence（K/L）分级量表评估膝关节X线片。通过高效液相色谱法（HPLC）测定以下二糖水平：DS（DSDeltaDi4S）、硫酸软骨素6-硫酸酯（CSDeltaDi6S）和硫酸软骨素4-硫酸酯（CSDeltaDi4S）。采用夹心ELISA法测定软骨寡聚基质蛋白（COMP）浓度。0级和I级OA中DSDeltaDi4S水平显著高于II级（P = 0.0458）、III级（P < 0.0001）和IV级（P < 0.0001），并且我们发现DSDeltaDi4S水平与CSDeltaDi6S水平（P < 0.0001，r = 0.705）、CSDeltaDi4S水平（P < 0.0001，r = 0.750）和COMP水平（P < 0.0001，r = 0.699）之间存在强相关性。我们得出结论，DSDeltaDi4S的存在反映了OA患者残余关节软骨中的蛋白聚糖代谢。这表明富含亮氨酸的小分子重复蛋白聚糖核心蛋白聚糖和双糖链蛋白聚糖（其含有DSDeltaDi4S链）的代谢与聚集蛋白聚糖的代谢相似。
