使用表面增强拉曼和超拉曼纳米传感器对细胞进行单光子和双光子激发光学pH探测。
One- and two-photon excited optical ph probing for cells using surface-enhanced Raman and hyper-Raman nanosensors.
作者信息
Kneipp Janina, Kneipp Harald, Wittig Burghardt, Kneipp Katrin
机构信息
Harvard University Medical School, Wellman Center for Photomedicine, Boston, Massachusetts 02114, USA.
出版信息
Nano Lett. 2007 Sep;7(9):2819-23. doi: 10.1021/nl071418z. Epub 2007 Aug 16.
We demonstrate spatially resolved probing and imaging of pH in live cells by mobile and biocompatible nanosensors using surface-enhanced Raman scattering (SERS) of 4-mercaptobenzoic acid (pMBA) on gold nanoaggregates. Moreover, we also show that this concept of pH nanosensors can be extended to two-photon excitation by using surface-enhanced hyper-Raman scattering (SEHRS). In addition to the advantages of two-photon excitation, the SEHRS sensor enables measurements over a wide pH range without the use of multiple probes.
我们通过使用金纳米聚集体上4-巯基苯甲酸（pMBA）的表面增强拉曼散射（SERS），利用可移动且生物相容的纳米传感器，展示了对活细胞内pH值的空间分辨探测和成像。此外，我们还表明，通过使用表面增强超拉曼散射（SEHRS），这种pH纳米传感器的概念可以扩展到双光子激发。除了双光子激发的优点外，SEHRS传感器能够在不使用多种探针的情况下，在很宽的pH范围内进行测量。
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