Integrative Biomedical Materials and Nanomedicine Lab, Department of Medicine and Life Sciences, University Pompeu Fabra, Doctor Aiguader 88, 08003, Barcelona, Spain.
Sci Rep. 2022 May 28;12(1):8977. doi: 10.1038/s41598-022-12989-6.
Biosensors, especially those with a SERS readout, are required for an early and precise healthcare diagnosis. Unreproducible SERS platforms hamper clinical SERS. Here we report a synthetic procedure to obtain stabile, reproducible and robust highly-SERS performing nanocomposites for labelling. We controlled the NPs agglomeration and codification which resulted in an increased number of hot spots, thus exhibiting reproducible and superior Raman enhancement. We studied fundamental aspects affecting the plasmonic thiol bond resulting in pH exhibiting a determining role. We validated their biosensing performance by designing a SERS-based detection assay model for SARS-CoV-2. The limit of detection of our assay detecting the spike RBD was below 10 ng/mL.
生物传感器，特别是那些具有 SERS 读出功能的生物传感器，是早期、准确的医疗诊断所必需的。不可重复的 SERS 平台阻碍了临床 SERS 的发展。在这里，我们报告了一种获得稳定、可重复和强大的高 SERS 纳米复合材料进行标记的合成方法。我们控制了 NPs 的聚集和编码，从而增加了热点的数量，从而表现出可重复和优异的拉曼增强。我们研究了影响等离子体硫醇键的基本方面，结果表明 pH 值起着决定性的作用。我们通过设计一种基于 SERS 的 SARS-CoV-2 检测分析模型来验证它们的生物传感性能。我们的分析方法检测刺突 RBD 的检测限低于 10ng/mL。
