Carlisle M S, McGregor D D, Appleton J A
James A. Baker Institute for Animal Health, Department of Microbiology, Immunology and Parasitology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
Immunology. 1991 Nov;74(3):546-51.
Entrapment of muscle larvae (ML) occurred in vitro when antibodies specific for Trichinella spiralis were added directly to intestinal mucus from normal non-immunized rats or when mucus was collected from pups suckling a T. spiralis-infected dam. Normal rat serum immunoglobulins failed to promote mucus entrapment and complement did not appear to play a part in the entrapment process. Differences were not observed in the efficiency of entrapment of ML by mucus harvested from different regions of the small intestine. Employing a panel of monoclonal antibodies (mAb) specific for excretory-secretory antigen (ESA), we attempted to dissociate antibody-mediated protection from mucus entrapment. We assessed mucus entrapment and rapid expulsion by these mAb in vivo, and observed protection in the absence of significant, immediate mucus entrapment in two cases. In addition, we measured mucus entrapment of ML in two in vitro assays. One assay employed intestinal mucus harvested from pups suckling dams that had been injected i.v. with a mAb. Results confirmed those obtained in vivo and indicated that antibodies were present in the intestinal lumina of passively immunized pups. In the second in vitro assay, mAb were added individually to mucus from pups suckling non-immunized dams. Results from these assays suggested that certain antibody isotypes may be processed in vivo in ways that influence, either positively or negatively, their abilities to cause mucus entrapment.
当将针对旋毛虫的特异性抗体直接添加到正常未免疫大鼠的肠道黏液中，或者当从吸食感染旋毛虫的母鼠乳汁的幼鼠收集黏液时，肌肉幼虫（ML）会在体外发生滞留。正常大鼠血清免疫球蛋白未能促进黏液滞留，并且补体似乎在滞留过程中不起作用。从小肠不同区域收集的黏液对ML的滞留效率未观察到差异。利用一组针对排泄分泌抗原（ESA）的单克隆抗体（mAb），我们试图将抗体介导的保护与黏液滞留区分开来。我们在体内评估了这些mAb对黏液滞留和快速排出的作用，并观察到在两例中存在保护作用但没有明显的立即黏液滞留现象。此外，我们在两种体外试验中测量了ML的黏液滞留情况。一种试验使用从静脉注射mAb的母鼠所哺育的幼鼠收集的肠道黏液。结果证实了在体内获得的结果，并表明被动免疫幼鼠的肠腔中存在抗体。在第二种体外试验中，将mAb分别添加到吸食未免疫母鼠乳汁的幼鼠的黏液中。这些试验的结果表明，某些抗体同种型在体内可能会以正向或负向影响其导致黏液滞留能力的方式进行加工。
