Shandra A A, Lobenko A A, Godlevsky L S, Mazarati A M, Bandyopadhyay A A
Department of Normal Physiology, N I Pirogov Medical Institute, Odessa, USSR.
Indian J Exp Biol. 1991 Aug;29(8):755-7.
Repeated picrotoxin administration (ip) in subthreshold doses in rats resulted in kindling of generalized seizures. Decrease of locomotor activity in kindled rats occurred in interictal periods. Intra-cerebroventricular microinjection to intact recipients of cerebrospinal fluid (CSF) of kindled but not intact rats or those after acute picrotoxin-induced convulsions, induced a decrease of locomotor activity and severity of acute picrotoxin induced seizures. These effects of CSF were blocked by naloxone pretreatment and were absent after injection of CSF to which protease inhibitors were not added. It is concluded that the release of endogenous opioid peptide substance(s) takes place in CSF of kindled animals which cause the interictal decrease of locomotor activity and may play the role of endogenous anticonvulsive factors controlling epileptic activity induction.
对大鼠反复腹腔注射阈下剂量的印防己毒素会引发全身性癫痫发作的点燃效应。在发作间期，点燃大鼠的运动活动会减少。向未点燃的正常大鼠或急性印防己毒素诱发惊厥后的大鼠脑室内微量注射点燃大鼠而非正常大鼠的脑脊液，会导致运动活动减少以及急性印防己毒素诱发惊厥的严重程度降低。脑脊液的这些作用可被纳洛酮预处理阻断，且在注射未添加蛋白酶抑制剂的脑脊液后不会出现。研究得出结论，内源性阿片肽物质在点燃动物的脑脊液中释放，这会导致发作间期运动活动减少，并可能在内源性抗惊厥因子控制癫痫活动诱发过程中发挥作用。
