微束耦合毛细管电泳
Microbeam-coupled capillary electrophoresis.
作者信息
Garty G, Ehsan M U, Buonanno M, Yang Z, Sweedler J V, Brenner D J
机构信息
Radiological Research Accelerator Facility, Columbia University, P.O. Box 21, Irvington, NY 10533, USA
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801, USA.
出版信息
Radiat Prot Dosimetry. 2015 Sep;166(1-4):188-91. doi: 10.1093/rpd/ncv148. Epub 2015 Apr 12.
Within the first few microseconds following a charged particle traversal of a cell, numerous oxygen and nitrogen radicals are formed along the track. Presented here is a method, using capillary electrophoresis, for simultaneous measurement, within an individual cell, of specific reactive oxygen species, such as the superoxide radical ([Formula: see text]) as well as the native and oxidised forms of glutathione, an ubiquitous anti-oxidant that assists the cell in coping with these species. Preliminary data are presented as well as plans for integrating this system into the charged particle microbeam at Columbia University.
在带电粒子穿过细胞后的最初几微秒内，沿轨迹会形成大量氧自由基和氮自由基。本文介绍了一种利用毛细管电泳在单个细胞内同时测量特定活性氧物种的方法，例如超氧阴离子自由基（[公式：见正文]）以及谷胱甘肽的天然形式和氧化形式，谷胱甘肽是一种普遍存在的抗氧化剂，可帮助细胞应对这些物种。文中还展示了初步数据以及将该系统整合到哥伦比亚大学带电粒子微束中的计划。
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