猫传染性腹膜炎病毒在猫血液单核细胞和克兰德尔猫肾细胞中的附着与内化

Attachment and internalization of feline infectious peritonitis virus in feline blood monocytes and Crandell feline kidney cells.

作者信息

Van Hamme Evelien, Dewerchin Hannah L, Cornelissen Els, Nauwynck Hans J

机构信息

Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.

出版信息

J Gen Virol. 2007 Sep;88(Pt 9):2527-2532. doi: 10.1099/vir.0.82991-0.

DOI:10.1099/vir.0.82991-0

PMID:17698663

Abstract

In this study, kinetics of attachment and internalization of feline infectious peritonitis virus (FIPV) serotype I strain Black and serotype II strain 79-1146 were determined in feline monocytes from two cats and in Crandell feline kidney (CrFK) cells. Attached FIPV I (Black) particles were observed on almost all monocytes. Within 1 h, 17 particles were bound per cell and, within 1 min, 89 % of the bound particles were internalized. For FIPV II (79-1146), attachment was observed on 66 and 95 % of all monocytes from the two cats. After 1 h, respectively five and 20 particles were bound per cell (all cells considered). Within 1 min, 60 % of the bound particles were internalized. Internalization in monocytes was efficient and proceeded via endocytosis. In CrFK cells, attachment and internalization were less efficient, especially for FIPV I (Black), so this cell line is not suitable for studying FIPV entry.

摘要

在本研究中，测定了I型猫传染性腹膜炎病毒（FIPV）毒株Black和II型毒株79 - 1146在两只猫的猫单核细胞以及Crandell猫肾（CrFK）细胞中的吸附和内化动力学。几乎在所有单核细胞上都观察到了吸附的FIPV I（Black）颗粒。1小时内，每个细胞结合17个颗粒，1分钟内，89%的结合颗粒被内化。对于FIPV II（79 - 1146），在两只猫的所有单核细胞中，分别有66%和95%观察到吸附。1小时后，每个细胞分别结合5个和20个颗粒（所有细胞均计算在内）。1分钟内，60%的结合颗粒被内化。单核细胞中的内化效率高，通过内吞作用进行。在CrFK细胞中，吸附和内化效率较低，尤其是对于FIPV I（Black），因此该细胞系不适合用于研究FIPV的进入。