Eller Leigh A, Eller Michael A, Ouma Benson J, Kataaha Peter, Bagaya Bernard S, Olemukan Robert L, Erima Simon, Kawala Lilian, de Souza Mark S, Kibuuka Hannah, Wabwire-Mangen Fred, Peel Sheila A, O'Connell Robert J, Robb Merlin L, Michael Nelson L
Makerere University Walter Reed Project, Kampala, Uganda.
J Clin Microbiol. 2007 Oct;45(10):3281-5. doi: 10.1128/JCM.00894-07. Epub 2007 Aug 15.
The use of rapid tests for human immunodeficiency virus (HIV) has become standard in HIV testing algorithms employed in resource-limited settings. We report an extensive HIV rapid test validation study conducted among Ugandan blood bank donors at low risk for HIV infection. The operational characteristics of four readily available commercial HIV rapid test kits were first determined with 940 donor samples and were used to select a serial testing algorithm. Uni-Gold Recombigen HIV was used as the screening test, followed by HIV-1/2 STAT-PAK for reactive samples. OraQuick HIV-1 testing was performed if the first two test results were discordant. This algorithm was then tested with 5,252 blood donor samples, and the results were compared to those of enzyme immunoassays (EIAs) and Western blotting. The unadjusted algorithm sensitivity and specificity were 98.6 and 99.9%, respectively. The adjusted sensitivity and specificity were 100 and 99.96%, respectively. This HIV testing algorithm is a suitable alternative to EIAs and Western blotting for Ugandan blood donors.
在资源有限环境中使用的艾滋病毒检测算法里，采用快速检测法检测人类免疫缺陷病毒（HIV）已成为标准做法。我们报告了一项针对乌干达血库中HIV感染低风险献血者开展的广泛的HIV快速检测验证研究。首先用940份献血者样本确定了四种现成商用HIV快速检测试剂盒的操作特性，并据此选择了一种系列检测算法。使用Uni-Gold Recombigen HIV作为筛查检测，反应性样本随后采用HIV-1/2 STAT-PAK检测。如果前两个检测结果不一致，则进行OraQuick HIV-1检测。然后用5252份献血者样本对该算法进行检测，并将结果与酶免疫测定（EIA）和蛋白质印迹法的结果进行比较。未经调整的算法灵敏度和特异性分别为98.6%和99.9%。调整后的灵敏度和特异性分别为100%和99.96%。对于乌干达献血者而言，这种HIV检测算法是替代EIA和蛋白质印迹法的合适选择。
