基于邻苯二甲醛和3-巯丙基三乙氧基硅烷的多巴胺荧光测定方法的开发。

Development of Fluorescence-Based Method for Dopamine Determination Using o-Phthaldialdehyde and 3-Mercaptopropyltriethoxysilane.

作者信息

Sliesarenko Valeriia, Krstić Marijana, Bren Urban, Lobnik Aleksandra

机构信息

Institute for Environmental Protection and Sensors, IOS Ltd., 7 Beloruska Str., SI-2000 Maribor, Slovenia.

Faculty of Chemistry and Chemical Engineering, University of Maribor, 17 Smetanova Str., SI-2000 Maribor, Slovenia.

出版信息

Sensors (Basel). 2025 Mar 11;25(6):1729. doi: 10.3390/s25061729.

DOI:10.3390/s25061729

PMID:40292813

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11946605/

Abstract

Nanomaterials and sensors play an important role in modern technologies, including medical diagnostics and biochemical research. This work presents the possibility of using o-Phthaldialdehyde (OPA) in combination with 3-mercaptopropyltriethoxysilane (MPTES) to develop a dopamine-responsive sensor. During the experiment, these materials were used at different pH and ratios to determine the optimal parameters for obtaining high fluorescence intensity of the reaction product. The data obtained demonstrate a linear relationship between the fluorescence response (λ/λ = 340/460 nm) of OPA/MPTES and dopamine concentration in the range of 0.1-3.0 µM at a pH of 8, and the detection limit was 8.7 nM. The obtained results confirm the potential of OPA/MPTES as a sensing component for the detection of dopamine.

摘要

纳米材料和传感器在现代技术中发挥着重要作用，包括医学诊断和生化研究。这项工作展示了使用邻苯二甲醛（OPA）与3-巯基丙基三乙氧基硅烷（MPTES）结合开发多巴胺响应传感器的可能性。在实验过程中，这些材料在不同的pH值和比例下使用，以确定获得反应产物高荧光强度的最佳参数。获得的数据表明，在pH值为8时，OPA/MPTES的荧光响应（λ/λ = 340/460 nm）与多巴胺浓度在0.1 - 3.0 µM范围内呈线性关系，检测限为8.7 nM。所得结果证实了OPA/MPTES作为检测多巴胺的传感组件的潜力。