Liu Wen-hu, Hsu Cheng-chin, Yin Mei-chin
Department of Nutritional Science, Chung Shan Medical University, Taichung City, Taiwan, ROC.
Phytother Res. 2008 Jan;22(1):53-7. doi: 10.1002/ptr.2259.
The in vitro inhibitory effects of diallyl disulphide (DADS), diallyl trisulphide (DAT), roselle calyx extract and protocatechuic acid (PA) on the growth of Helicobacter pylori (15 susceptible, 11 clarithromycin-resistant and 9 metronidazole-resistant strains) were studied. The inhibition zone was determined after each agent had been heated at 25, 60, 100 degrees C for 60 min. The minimal inhibitory concentration (MIC) of each agent was determined by the tube dilution assay. The results showed that heat treatment did not affect the anti-H. pylori activity of DADS, DAT, roselle calyx extract and PA, and the MIC values of these agents against test H. pylori strains were in the range 8-64 mg/L. The time-kill study assay for DAT and PA at 1x MIC was monitored in Muller Hinton broth supplemented with 10% horse blood or mice stomach homogenate. Both DAT and PA inhibited the growth of all test H. pylori in broth and mice stomach homogenate (p < 0.05); however, the inhibitory effects of these two agents were less in mice stomach homogenate than in broth (p < 0.05). DAT at 4, 6, 8, 10, 12, 14 mg/L and PA at 8, 16, 24, 32, 40, 48 mg/L were used for urease activity assay. These two agents significantly reduced urease activity of test H. pylori strains (p < 0.05), in which DAT and PA at 1x MIC reduced the urease activity of H. pylori to 70% and 40%, respectively. These agents, based on their lower MIC values and heat tolerance, might be useful in the prevention or therapy of H. pylori.
研究了二烯丙基二硫化物（DADS）、二烯丙基三硫化物（DAT）、玫瑰茄花萼提取物和原儿茶酸（PA）对幽门螺杆菌（15株敏感菌株、11株克拉霉素耐药菌株和9株甲硝唑耐药菌株）生长的体外抑制作用。每种试剂在25、60、100℃加热60分钟后测定抑菌圈。通过试管稀释法测定每种试剂的最低抑菌浓度（MIC）。结果表明，热处理不影响DADS、DAT、玫瑰茄花萼提取物和PA的抗幽门螺杆菌活性，这些试剂对受试幽门螺杆菌菌株的MIC值在8 - 64 mg/L范围内。在补充有10%马血或小鼠胃匀浆的Muller Hinton肉汤中监测DAT和PA在1x MIC时的时间杀菌研究试验。DAT和PA均抑制肉汤和小鼠胃匀浆中所有受试幽门螺杆菌的生长（p < 0.05）；然而，这两种试剂在小鼠胃匀浆中的抑制作用比在肉汤中弱（p < 0.05）。使用4、6、8、10、12、14 mg/L的DAT和8、16、24、32、40、48 mg/L的PA进行脲酶活性测定。这两种试剂显著降低了受试幽门螺杆菌菌株的脲酶活性（p < 0.05），其中1x MIC的DAT和PA分别将幽门螺杆菌的脲酶活性降低到70%和40%。基于其较低的MIC值和耐热性，这些试剂可能对幽门螺杆菌的预防或治疗有用。
