Zhao Xuebing, Zhang Lihua, Liu Dehua
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Bioresour Technol. 2008 Jun;99(9):3729-36. doi: 10.1016/j.biortech.2007.07.016. Epub 2007 Aug 20.
In order to utilize and control the invasive weed, crofton weed (Eupatorium adenophorum Spreng), a potential pathway was proposed by using it as a feedstock for production of fermentable sugars. Three chemical pretreatment methods were used for improving enzymatic saccharification of the weed stem. Mild H2SO4 pretreatment could obtain a relatively high yield of sugars in the pretreatment (32.89%, based on initial holocellulose), however, it led to only a slight enhancement of enzymatic digestibility. NaOH pretreatment could obtain a higher enzymatic conversion ratio of cellulose compared with H2SO4 pretreatment. Peracetic acid (PAA) pretreatment seemed to be the most effective for improving enzymatic saccharification of the weed stem in the three chemical pretreatment methods under the same conditions. The conversion ratio of cellulose in the sample pretreated by PAA under the "optimal" condition was increased to 50% by cellulase loading of 80 FPU/g cellulose for 72 h incubation. A number of empirical quadratic models were successfully developed according to the experimental data to predict the yield of sugar and degree of delignification.
为了利用和控制入侵杂草紫茎泽兰，提出了一条将其用作生产可发酵糖原料的潜在途径。采用三种化学预处理方法来提高该杂草茎的酶解糖化效果。温和的硫酸预处理在预处理过程中能获得相对较高的糖产量（基于初始全纤维素为32.89%），然而，它仅使酶解消化率略有提高。与硫酸预处理相比，氢氧化钠预处理能获得更高的纤维素酶转化比率。在相同条件下的三种化学预处理方法中，过氧乙酸（PAA）预处理似乎对提高杂草茎的酶解糖化最为有效。在“最佳”条件下，经PAA预处理的样品中，通过80 FPU/g纤维素的纤维素酶负载量孵育72小时，纤维素转化率提高到了50%。根据实验数据成功建立了多个经验二次模型，以预测糖产量和脱木质素程度。
