Wei Yun, Gao Yali, Zhang Kai, Ito Yoichiro
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China.
J Liq Chromatogr Relat Technol. 2010 Jan 1;33(6):837-845. doi: 10.1080/10826071003684471.
Preparative high-speed countercurrent chromatography (HSCCC) was successfully used for isolation and purification of caffeic acid from Eupatorium adenophorum Spreng with a solvent system composed of ethyl acetate-methanol-water at a volume ratio of 10:1:10, v/v. Using a preparative unit of the HSCCC centrifuge, about a 938 mg amount of the crude extract was separated, yielding 63.2 mg of caffeic acid at purity of 96.0%. Then, the anti-microbial and anti-virus drug caffeic acid (C(9)H(8)O(4)) was intercalated into layered double hydroxides for the first time by anion exchange under a nitrogen atmosphere. The product caffeic acid-LDH has been characterized by powder X-ray diffraction (XRD), Fourier transform-infrared (FT-IR), Scanning electron micrographs (SEM), indicating that the drug has been successfully intercalated into LDH.
制备型高速逆流色谱（HSCCC）成功用于从紫茎泽兰中分离纯化咖啡酸，所用溶剂系统为体积比10:1:10（v/v）的乙酸乙酯 - 甲醇 - 水。使用HSCCC离心机的制备单元，分离出约938 mg粗提取物，得到纯度为96.0%的咖啡酸63.2 mg。然后，首次在氮气气氛下通过阴离子交换将抗菌抗病毒药物咖啡酸（C(9)H(8)O(4)）插入层状双氢氧化物中。产物咖啡酸 - LDH已通过粉末X射线衍射（XRD）、傅里叶变换红外光谱（FT - IR）、扫描电子显微镜（SEM）进行表征，表明该药物已成功插入LDH中。
