He Xiaowei, Guo Qiuhua, Peng Yunping, Huang Qiang
College of Light Industy and Food Science, South China University of Technology, Guangzhou 510640,China.
Wei Sheng Yan Jiu. 2007 May;36(3):388-90.
Microcystins (MCs) may be group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae). Their toxicity could be associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). There are some methods for analyzing and detecting MCs in aquatic environment, such as HPLC, LC-MS, PPIA, and ELISA. This review introduces advances on MCs determined by enzyme-linked immunosorbent assay (ELISA), and estimates the prospective development of ELISA for monitoring MCs in natural waters.
微囊藻毒素（MCs）可能是由常见蓝藻（蓝绿藻）产生的一组密切相关的有毒环状七肽。它们的毒性可能与细胞内1型和2型蛋白磷酸酶（分别为PP1和PP2A）的特异性抑制有关。在水生环境中分析和检测微囊藻毒素有一些方法，如高效液相色谱法（HPLC）、液相色谱-质谱联用（LC-MS）、蛋白磷酸酶抑制活性分析（PPIA）和酶联免疫吸附测定（ELISA）。本文综述了酶联免疫吸附测定法（ELISA）测定微囊藻毒素的研究进展，并对ELISA法监测天然水体中微囊藻毒素的未来发展进行了评估。
