[酶联免疫吸附测定法在微囊藻毒素检测中的应用]
[Application of ELISA for microcystins detection].
作者信息
He Xiaowei, Guo Qiuhua, Peng Yunping, Huang Qiang
机构信息
College of Light Industy and Food Science, South China University of Technology, Guangzhou 510640,China.
出版信息
Wei Sheng Yan Jiu. 2007 May;36(3):388-90.
Microcystins (MCs) may be group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae). Their toxicity could be associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). There are some methods for analyzing and detecting MCs in aquatic environment, such as HPLC, LC-MS, PPIA, and ELISA. This review introduces advances on MCs determined by enzyme-linked immunosorbent assay (ELISA), and estimates the prospective development of ELISA for monitoring MCs in natural waters.
微囊藻毒素（MCs）可能是由常见蓝藻（蓝绿藻）产生的一组密切相关的有毒环状七肽。它们的毒性可能与细胞内1型和2型蛋白磷酸酶（分别为PP1和PP2A）的特异性抑制有关。在水生环境中分析和检测微囊藻毒素有一些方法，如高效液相色谱法（HPLC）、液相色谱-质谱联用（LC-MS）、蛋白磷酸酶抑制活性分析（PPIA）和酶联免疫吸附测定（ELISA）。本文综述了酶联免疫吸附测定法（ELISA）测定微囊藻毒素的研究进展，并对ELISA法监测天然水体中微囊藻毒素的未来发展进行了评估。
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