Ferranti Pasquale, Fabbrocino Serena, Cerulo Maria Grazia, Bruno Milena, Serpe Luigi, Gallo Pasquale
University of Naples, Portici, NA, Italy.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Dec;25(12):1530-7. doi: 10.1080/02652030802136196.
Cyanobacteria (blue-green algae) cause blooms in eutrophic lakes and drinking water reservoirs. They also produce biotoxins, including microcystins (MCs), highly toxic cyclic heptapeptides that cause poisoning in animals and human. In this paper, we present a method for the analysis of four MCs by ion trap LC-MS and MALDI-TOF/MS. The data are compared to evaluate the performance and reliability of the different MS detection systems. The method was applied to the analysis of water and algae samples from Lake Averno, near Naples, as a consequence of a cyanobacteria bloom. The analysis of algae cell extracts showed no contamination by known microcystins, but the three main substances were detected. MALDI-TOF/MS was successful for screening of the biotoxins in the samples, identifying anabaenopeptin B and anabaenopeptin F as the major contaminants on the basis of literature mass spectrometry data. The structure of the third compound was not identified and is under further investigation. The method could characterise the biotoxins produced in Lake Averno for evaluating health risks related to their presence.
蓝藻（蓝绿藻）会在富营养化湖泊和饮用水水库中大量繁殖。它们还会产生生物毒素，包括微囊藻毒素（MCs），这是一类剧毒的环状七肽，可导致动物和人类中毒。在本文中，我们介绍了一种通过离子阱液相色谱 - 质谱联用仪（LC-MS）和基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF/MS）分析四种微囊藻毒素的方法。对数据进行比较，以评估不同质谱检测系统的性能和可靠性。由于蓝藻大量繁殖，该方法被应用于分析那不勒斯附近阿韦尔诺湖的水和藻类样本。藻类细胞提取物的分析表明，样本未受到已知微囊藻毒素的污染，但检测到了三种主要物质。MALDI-TOF/MS成功地筛选了样本中的生物毒素，根据文献质谱数据确定阿那巴肽B和阿那巴肽F为主要污染物。第三种化合物的结构尚未确定，正在进一步研究中。该方法可以对阿韦尔诺湖产生的生物毒素进行表征，以评估与其存在相关的健康风险。
